DDX6 Antibody

Novus Biologicals | Catalog # NB200-191

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human, Mouse, Rat

Predicted:

Chicken (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DDX6 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by NB200-191 maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).

Reactivity Notes

Hamster reactivity reported from a verified customer reivew.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX6 Antibody

Western Blot: DDX6 Antibody [NB200-191]

Western Blot: DDX6 Antibody [NB200-191]

DDX6-Antibody-Western-Blot-NB200-191-img0014.jpg
Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-191]

Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-191]

Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunoprecipitation: DDX6 Antibody [NB200-191]

Immunoprecipitation: DDX6 Antibody [NB200-191]

Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
DDX6 Antibody

Western Blot: DDX6 Antibody [NB200-191] -

Western Blot: DDX6 Antibody [NB200-191] - Staufen1 protein but not mRNA steady-state levels are increased in neurodegenerative disease cells & tissues. Western blot analysis of SCA2- FBs (a) & LBCs (b) show increased STAU1 levels compared with normal controls. DDX6 levels are unchanged. HD & SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal & five SCA2 FBs, & two normal & three SCA2 LBCs were used. c, d Western blot analyses of ATXN2Q127 (c) & BAC-Q72 (d) mouse cerebellar extracts (24 weeks of age) showing increased Stau1 levels compared with wild-type or BAC-Q22 controls (n = 2–3 animals per group). e Western blot of FB extracts from an ALS patient with the TDP-43G298S mutation show increased STAU1 levels. beta -Actin was used as loading control & representative blots of three independent experiments are shown. f–hSTAU1 RNA levels are unaltered in SCA2 & ALS cells & SCA2 mice. qRT-PCR analyses of STAU1 mRNA in SCA2 FBs & ALS FB with TDP-43G298S mutation (f) or SCA2 LBCs (g). h qRT-PCR analyses of cerebellar RNAs from ATXN2Q127 & BAC-Q72 mice compared to wild-type littermates (24 weeks of age; n = animals per group). Gene expression levels were normalized to Actb Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DDX6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
ICC/IF was reported in scientific literature (PMID: 25800057) and a verified customer review. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB200-191 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX6

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Alternate Names

ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), EC 3.6.1, EC 3.6.4.13, FLJ36338, HLR2DEAD box-6, Oncogene RCK, probable ATP-dependent RNA helicase DDX6, RCKP54

Entrez Gene IDs

1656 (Human)

Gene Symbol

DDX6

UniProt

P26196 (Human)

Additional DDX6 Products

Product Documents for DDX6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DDX6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DDX6 Antibody (1)

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1 Customer Rating
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Customer Images

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  • DDX6 Antibody
    Name: Lifeng Liu
    Application: Immunofluorescence and microscopy
    Sample Tested: MEFs, BHK (Baby Hamster Kidney fibroblasts), U2OS cells and HOS human osteosarcoma cell line
    Species: Human, Mouse and Hamster
    Verified Customer | Posted 08/22/2017
    In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs).
    Cells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.
    DDX6 Antibody NB200-191

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