Desmoglein-3 Antibody (5H10) - BSA Free

Novus Biologicals | Catalog # NBP1-78984

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Cytometric Bead Assay Standard

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H10

Format

BSA Free
View all Desmoglein-3 Primary Antibodies »

Product Specifications

Immunogen

The extracellular domain of human Desmoglein 3 [Swiss-Prot# P32926]

Localization

Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmoglein-3 Antibody (5H10) - BSA Free

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Desmoglein-3-Antibody-5H10-Western-Blot-NBP1-78984-img0008.jpg
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.
Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.
Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984.
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in oral HNSCC cells fixed with 1% formaldehyde permeabilized in 0.1% Triton X-100.
Desmoglein-3 Antibody (5H10) - BSA Free

Desmoglein-3 Antibody (5H10) in A431 Human Cell Line.

Desmoglein-3 Antibody (5H10) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-Desmoglein-3 Antibody (5H10) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP1-78984AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Desmoglein-3 Antibody (5H10) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 15588331)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
In Western blot, a band can be seen at approximately 130 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP1-78984 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Alternate Names

CDHF6, Desmoglein3, DSG3

Entrez Gene IDs

1830 (Human)

Gene Symbol

DSG3

UniProt

P32926

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (5H10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Desmoglein-3 Antibody (5H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Desmoglein-3 Antibody (5H10) - BSA Free

Customer Reviews for Desmoglein-3 Antibody (5H10) - BSA Free (1)

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Customer Images

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  • Desmoglein-3 Antibody (5H10)
    Name: Qi Miao
    Application: Immunocytochemistry
    Sample Tested: Primary skin keratinocytes
    Species: Mouse
    Verified Customer | Posted 06/22/2017
    The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining.
    Desmoglein-3 Antibody (5H10) - BSA Free NBP1-78984

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Protocols

View specific protocols for Desmoglein-3 Antibody (5H10) - BSA Free (NBP1-78984):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures."


Immunohistochemistry-Paraffin Embedded Sections
Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.
Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures."

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FAQs

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