DGAT1 Antibody (SR1829) - BSA Free

Novus Biologicals | Catalog # NBP3-22666

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1829 expressed in HEK293

Format

BSA Free
View all DGAT1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human DGAT1 (Uniprot #: O75907)

Specificity

Detects endogenous levels of total DGAT1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody (SR1829) - BSA Free

Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] -

Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.

Applications for DGAT1 Antibody (SR1829) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:60

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGAT1

Diglyceride acyltransferase (DGAT) is a membrane-bound Sterol o-acyltransferase subfamily member. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. Specifically, it catalyzes the terminal step in triacylglycerol synthesis, using diacylglycerol (DAG) and fatty acyl CoA as substrates.

DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody (SR1829) - BSA Free

Certificate of Analysis

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Product Specific Notices for DGAT1 Antibody (SR1829) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

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Protocols

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FAQs

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