DHX36 Antibody - BSA Free

Novus Biologicals | Catalog # NB200-353

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX36 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 950 and the C-terminus (residue 1008) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 36 using the numbering given in entry NP_065916.1

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23651854).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX36 Antibody - BSA Free

Western Blot: DHX36 Antibody [NB200-353]

Western Blot: DHX36 Antibody [NB200-353]

Western Blot: DHX36 Antibody [NB200-353] - Whole cell lysate from NIH3T3 (50ug), TCKM (50ug) and Renca (50ug) cells. Antibodies: Affinity purified rabbit antiDHX36 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: DHX36 Antibody [NB200-353]

Immunohistochemistry: DHX36 Antibody [NB200-353]

Immunohistochemistry: DHX36 Antibody [NB200-353] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DHX36 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: DHX36 Antibody [NB200-353]

Western Blot: DHX36 Antibody [NB200-353]

Western Blot: DHX36 Antibody [NB200-353] - Detection of Human DHX36 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug) and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: DHX36 Antibody [NB200-353]

Immunoprecipitation: DHX36 Antibody [NB200-353]

Immunoprecipitation: DHX36 Antibody [NB200-353] - Detection of human DHX36 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 (lot NB200-353-2) used for IP at 6 ug/mg lysate. DHX36 was also immunoprecipitated by a previous lot (NB200-353-1) of this antibody. For blotting immunoprecipitated DHX36, NB200-353 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DHX36 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:5000-1:15000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX36

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Alternate Names

DDX36, DEAH (Asp-Glu-Ala-His) box polypeptide 36, DEAH box protein 36, EC 3.6.1, EC 3.6.4.13, G4 resolvase-1, KIAA1488G4R1, MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36, MLE-like protein 1, probable ATP-dependent RNA helicase DHX36, RNA helicase associated with AU-rich element ARE

Entrez Gene IDs

170506 (Human); 72162 (Mouse)

Gene Symbol

DHX36

UniProt

Q9H2U1 (Human)

Additional DHX36 Products

Product Documents for DHX36 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DHX36 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DHX36 Antibody - BSA Free

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Protocols

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