DHX36 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84286

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX36 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX36 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DHX36 Antibody [NBP1-84286]

Immunocytochemistry/ Immunofluorescence: DHX36 Antibody [NBP1-84286]

Immunocytochemistry/Immunofluorescence: DHX36 Antibody [NBP1-84286] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
DHX36 Antibody - BSA Free Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
DHX36 Antibody - BSA Free Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Staining of human placenta shows strong cytoplasmic positivity in decidual cells.
DHX36 Antibody - BSA Free Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
DHX36 Antibody - BSA Free Western Blot: DHX36 Antibody - BSA Free [NBP1-84286]

Western Blot: DHX36 Antibody - BSA Free [NBP1-84286]

Analysis in human cell line EFO-21.
DHX36 Antibody - BSA Free Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody - BSA Free [NBP1-84286]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for DHX36 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX36

DHX36, also known as Probable ATP-dependent RNA helicase DHX36, consists of three isoforms of sizes 114.7 kDa, 113.2 kDa, and 111.5 kDa and is involved in breaking down mRNA with the ARE element in their 3'-UTR strand. Current research links this protein to sex development and spermatogenesis. The protein interacts with RPS9, AGO1, UBC, AGO2, and PHB in the RNA degradation pathway.

Alternate Names

DDX36, DEAH (Asp-Glu-Ala-His) box polypeptide 36, DEAH box protein 36, EC 3.6.1, EC 3.6.4.13, G4 resolvase-1, KIAA1488G4R1, MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36, MLE-like protein 1, probable ATP-dependent RNA helicase DHX36, RNA helicase associated with AU-rich element ARE

Gene Symbol

DHX36

Additional DHX36 Products

Product Documents for DHX36 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DHX36 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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