DIAPH-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85217

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DIAPH-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQIKTLADDVRDRITSFRKSTVKKEKPLIQHPIDSQVAMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDMNLNEEKKAPLRNKDFTTKREMVVQYISAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIAPH-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.
Western Blot: DIAPH-2 Antibody [NBP1-85217]

Western Blot: DIAPH-2 Antibody [NBP1-85217]

Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: DIAPH-2 Antibody [NBP1-85217]

Immunocytochemistry/ Immunofluorescence: DIAPH-2 Antibody [NBP1-85217]

Immunocytochemistry/Immunofluorescence: DIAPH-2 Antibody [NBP1-85217] - Staining of human cell line U-251 MG shows localization to nucleoli, endoplasmic reticulum and vesicles. Antibody staining is shown in green.
Western Blot: DIAPH-2 Antibody [NBP1-85217]

Western Blot: DIAPH-2 Antibody [NBP1-85217]

Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.

Applications for DIAPH-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, WB reported in scientific literature (PMID: 27922008). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIAPH-2

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Entrez Gene IDs

1730 (Human)

Gene Symbol

DIAPH2

UniProt

O60879

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DIAPH-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DIAPH-2 Antibody - BSA Free

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Protocols

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