Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 611621
View all Digoxigenin Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Digoxigenin

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Digoxigenin Biotinylated Antibody

Digoxigenin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Digoxigenin in Mouse Brain.

Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using the Digoxigenin Biotinylated Monoclonal Antibody (brown; Catalog # BAM7520) at 3 µg/mL followed by Streptavidin-HRP incubation. Specific staining was localized to vasculature. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Digoxigenin Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Digoxigenin Biotinylated Antibody

For research use only

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Protocols

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