Detects mouse Netrin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximatley 25% cross-reactivity with recombinant human Netrin-4 and less than 1% cross-reactivity with recombinant mouse Netrin-1, recombinant chicken Netrin‑2, and recombinant mouse Netrin-G1a.
In a functional ELISA, 0.08-0.24 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse Netrin-4 (Catalog # 1132-N4) to immobilized Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Netrin‑4 in Mouse Brain. Netrin‑4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Mouse Netrin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29%, and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1-7).
McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
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