Mouse Netrin-4 Antibody

Catalog # Availability Size / Price Qty
AF1132
AF1132-SP
Netrin‑4 in Mouse Brain.
1 Image
Product Details
Citations (4)
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Mouse Netrin-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Netrin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximatley 25% cross-reactivity with recombinant human Netrin-4 and less than 1% cross-reactivity with recombinant mouse Netrin-1, recombinant chicken Netrin‑2, and recombinant mouse Netrin-G1a.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-4
Leu19-Val628
Accession # Q9JI33
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Netrin‑4 (Catalog # 1132-N4)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.08-0.24 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse Netrin-4 (Catalog # 1132-N4) to immobilized Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Netrin‑4 in Mouse Brain. View Larger

Netrin‑4 in Mouse Brain. Netrin‑4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Mouse Netrin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29%, and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1-7).

References
  1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
  2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
  3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
  4. Stein, E. et al. (2001) Science 291:1976.
  5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
  6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
  7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Entrez Gene IDs
59277 (Human); 57764 (Mouse)
Alternate Names
beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091

Product Datasheets

Citations for Mouse Netrin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B
    Authors: Toshihide Yamashita
    J. Exp. Med., 2016;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo
  2. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC OCT-embedded
  3. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  4. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
    Authors: Wang W, Reeves WB, Ramesh G
    Am. J. Physiol. Renal Physiol., 2008;294(4):F739-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen

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