Mouse Netrin-4 Antibody Summary
Accession # Q9JI33
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Netrin‑4 in Mouse Brain. Netrin‑4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Mouse Netrin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29%, and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1-7).
- McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
- Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
- Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
- Stein, E. et al. (2001) Science 291:1976.
- Koch, M. et al. (2000) J. Cell Biol. 151:221.
- Yin, Y. et al. (2000) Mech. Dev. 96:115.
- Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Citations for Mouse Netrin-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B
Authors: Toshihide Yamashita
J. Exp. Med., 2016;0(0):.
Sample Types: In Vivo
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
EMBO J, 2013;32(5):629-44.
Sample Types: Whole Cells
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Authors: Hoang S, Liauw J, Choi M
J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
Sample Types: Whole Tissue
Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
Authors: Wang W, Reeves WB, Ramesh G
Am. J. Physiol. Renal Physiol., 2008;294(4):F739-47.
Sample Types: Whole Tissue
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