Digoxigenin Antibody

Catalog # Availability Size / Price Qty
Digoxigenin in Mouse Brain.
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Product Details
Citations (2)
Supplemental Products

Digoxigenin Antibody Summary

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
Monoclonal Mouse IgG2A Clone # 611621
Protein A or G purified from hybridoma culture supernatant
KLH-coupled Digoxigenin
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Digoxigenin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Digoxigenin in Mouse Brain. Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using 25 µg/mL Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB7520) followed by incubation with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Tissue was counter-stained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
Alternate Names
DIG; Digoxigenin

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Citations for Digoxigenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Stress-induced plasticity of a CRH/GABA projection disrupts reward behaviors in mice
    Authors: MT Birnie, AK Short, GB de Carvalh, L Taniguchi, BG Gunn, AL Pham, CA Itoga, X Xu, LY Chen, SV Mahler, Y Chen, TZ Baram
    Nature Communications, 2023;14(1):1088.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Tau-induced nuclear envelope invagination causes a toxic accumulation of mRNA in Drosophila
    Authors: GL Cornelison, SA Levy, T Jenson, B Frost
    Aging Cell, 2018;0(0):e12847.
    Species: Drosophila
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Mouse IgG2A Isotype Control

Primary Antibodies

Reconstitution Buffers

Secondary Antibodies

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