Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Insect - Drosophila, Transgenic Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 611621
View all Digoxigenin Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Digoxigenin

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Digoxigenin Antibody

Digoxigenin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Digoxigenin in Mouse Brain.

Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using 25 µg/mL Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB7520) followed by incubation with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Tissue was counter-stained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Digoxigenin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Digoxigenin Antibody

For research use only

Citations for Digoxigenin Antibody

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Protocols

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