Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Novus Biologicals | Catalog # NBP1-47727

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6G7
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens dihydrofolate reductase (DHFR).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.
Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dihydrofolate reductase.
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [NBP1-47727]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.
Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] -

Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Left: lysate of glioblastoma xenograft. Non-treated. Right: methotrexate-treated lysate of glioblastoma xenograft. 10 ug each protein were loaded.Antibody dilution is 1/3000 in 2% BSA TBST. Image from verified customer review.

Applications for Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-47727 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Entrez Gene IDs

1719 (Human)

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for Dihydrofolate Reductase/DHFR Antibody (OTI6G7)

Customer Reviews for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) (1)

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Customer Images

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  • Dihydrofolate Reductase/DHFR Antibody (OTI6G7)
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: Human Xenograft Tumor
    Species: Human
    Verified Customer | Posted 06/23/2023
    left: lysate of glioblastoma xenograft. Non-treated. Right: methotrexate-treated lysate of glioblastoma xenograft. 10 ug each protein are loaded. antibody dilution is 1/3000 in 2% BSA TBST. Beautiful single band is appeared.
    Dihydrofolate Reductase/DHFR Antibody (OTI6G7) NBP1-47727

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