Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70569

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6G7

Format

Azide and BSA Free
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7)Azide and BSA Free [NBP2-70569]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7)Azide and BSA Free [NBP2-70569]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant
Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.
Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7)Azide and BSA Free [NBP2-70569]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7)Azide and BSA Free [NBP2-70569]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.

Applications for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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