Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31302

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Canine (98%), Chicken (93%), Porcine (99%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dihydrolipoamide Dehydrogenase/DLD Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dihydrolipoamide Dehydrogenase/DLD. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (94%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.
Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] -

Dihydrolipoamide Dehydrogenase/DLD antibody detects Dihydrolipoamide Dehydrogenase/DLD protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Dihydrolipoamide Dehydrogenase/DLD protein stained by Dihydrolipoamide Dehydrogenase/DLD antibody (NBP1-31302) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP1-31302 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dihydrolipoamide Dehydrogenase/DLD

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Gene Symbol

DLD

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

Citations for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Customer Reviews for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free (1)

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  • Name: Hongxia Hu
    Application: Western Blot
    Sample Tested: Hela whole cell lysate, Sample Amount: 10ug
    Species: Human
    Verified Customer | Posted 01/09/2011
    Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free NBP1-31302

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Protocols

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