Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Novus Biologicals | Catalog # NB300-916

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence TCCSVLINKKVDS corresponding to C-Terminus according to NP_036269.1, NP_001127917.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic, nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of HepG2 (A) and LNCaP (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

0.1 - 0.3 ug/mL

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dimethylarginine Dimethylaminohydrolase 1/DDAH1

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Alternate Names

DDAHI, Dimethylargininase-1

Entrez Gene IDs

23576 (Human)

Gene Symbol

DDAH1

UniProt

NP_036269.1 (Human)

Additional Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Products

Product Documents for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Customer Reviews for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

There are currently no reviews for this product. Be the first to review Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody and earn rewards!

Have you used Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies