Dishevelled-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32388

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human

Predicted:

Bovine (99%), Rat (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dishevelled-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dishevelled-2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (80%), Xenopus laevis (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dishevelled-2 Antibody - BSA Free

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Dishevelled-2-Antibody-Western-Blot-NBP1-32388-img0007.jpg
Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388]

Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388]

Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388] - Paraffin-embedded lung SCC, using antibody at 1:100 dilution.
Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Sample(30 ug whole cell lysate)A: MOLT4 )B: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.
Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Whole cell extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Dishevelled-2 Antibody

Immunohistochemistry: Dishevelled-2 Antibody [NBP1-32388] -

Immunohistochemistry: Dishevelled-2 Antibody [NBP1-32388] - cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled-2 antibody [N2C2], Internal (NBP1-32388) at a 1:200 dilution.

Applications for Dishevelled-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dishevelled-2

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Alternate Names

Dishevelled2, DVL2

Gene Symbol

DVL2

Additional Dishevelled-2 Products

Product Documents for Dishevelled-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dishevelled-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Wnt Family

Citations for Dishevelled-2 Antibody - BSA Free

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Protocols

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FAQs

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