Dkk-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80843

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dkk-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYLAASW

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dkk-3 Antibody - BSA Free

Western Blot: Dkk-3 Antibody [NBP1-80843]

Western Blot: Dkk-3 Antibody [NBP1-80843]

Western Blot: Dkk-3 Antibody [NBP1-80843] - Analysis in control (vector only transfected HEK293T lysate) and DKK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody [NBP1-80843]

Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody [NBP1-80843]

Immunocytochemistry/Immunofluorescence: Dkk-3 Antibody [NBP1-80843] - Staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843]

Immunohistochemistry-Paraffin: Dkk-3 Antibody [NBP1-80843] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Applications for Dkk-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dkk-3

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Entrez Gene IDs

27122 (Human)

Gene Symbol

DKK3

UniProt

Q9UBP4

Additional Dkk-3 Products

Product Documents for Dkk-3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Dkk-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dkk-3 Antibody - BSA Free

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Protocols

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FAQs

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