DLL3 Antibody (1E1C7)

Novus Biologicals | Catalog # NBP3-57405

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E1C7
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Product Specifications

Immunogen

Recombinant protein (or fragment).This information is considered to be commercially sensitive. (Swissprot# Q9NYJ7)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer, pancreatic endocrine tumor, small cell lung cancer, malignant melanoma

Positive Controls
Pancreatic endocrine tumor, small cell lung cancer, malignant melanoma

Negative Controls
Fallopian tube, Lung, Kidney, Placenta,, Liver, Thyroid,, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma

Applications for DLL3 Antibody (1E1C7)

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry-Paraffin

1:200 - 1:800

Immunoprecipitation

0.15 ug - 0.3 ug antibody for 200 ug - 400 ug extracts of whole cells

Western Blot

1:2000 - 1:10000
Application Notes
For high-ratio antibody dilutions (greater than 1:10000), a sequential dilution method is strongly recommended to ensure measurement accuracy.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DLL3

The DLL3 gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostos

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Gene Symbol

DLL3

Additional DLL3 Products

Product Documents for DLL3 Antibody (1E1C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DLL3 Antibody (1E1C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail