DLL3 Antibody (1E1C7)
Novus Biologicals | Catalog # NBP3-57405
Recombinant Monoclonal Antibody
![DLL3 Antibody (1E1C7) Immunocytochemistry/ Immunofluorescence: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-immunocytochemistry-immunofluorescence-639289274432681012.jpg "Immunocytochemistry/ Immunofluorescence: DLL3 Antibody (1E1C7) [NBP3-57405]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E1C7
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Product Specifications
Immunogen
Recombinant protein (or fragment).This information is considered to be commercially sensitive. (Swissprot# Q9NYJ7)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain
Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer, pancreatic endocrine tumor, small cell lung cancer, malignant melanoma
Positive Controls
Pancreatic endocrine tumor, small cell lung cancer, malignant melanoma
Negative Controls
Fallopian tube, Lung, Kidney, Placenta,, Liver, Thyroid,, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma
Scientific Data Images for DLL3 Antibody (1E1C7)
Immunocytochemistry/ Immunofluorescence: DLL3 Antibody (1E1C7) [NBP3-57405]
Confocal imaging of TT cells and BxPC-3 (negative) cells using NBP3-57405 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.![DLL3 Antibody (1E1C7) Immunoprecipitation: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-immunoprecipitation-639289274432776268.jpg "Immunoprecipitation: DLL3 Antibody (1E1C7) [NBP3-57405]")
Immunoprecipitation: DLL3 Antibody (1E1C7) [NBP3-57405]
Immunoprecipitation of DLL3 from 300 µg extracts of TT cells was performed using 0.15 ug NBP3-57405. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using NBP3-57405 at a dilution of 1:5000.![DLL3 Antibody (1E1C7) Western Blot: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-western-blot-639289274432830317.jpg "Western Blot: DLL3 Antibody (1E1C7) [NBP3-57405]")
Western Blot: DLL3 Antibody (1E1C7) [NBP3-57405]
Western blot analysis of various lysates using NBP3-57405 at 1:5000 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Negative control (NC): BxPC-3. Exposure time: 10 s.![DLL3 Antibody (1E1C7) Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-immunohistochemistry-paraffin-639289274468006381.jpg "Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]")
Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]
Immunohistochemistry analysis of paraffin-embedded Human malignant melanoma tissue using NBP3-57405 at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.![DLL3 Antibody (1E1C7) Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-immunohistochemistry-paraffin-639289274468689348.jpg "Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]")
Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]
Immunohistochemistry analysis of paraffin-embedded Human pancreatic cancer tissue using NBP3-57405 at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.![DLL3 Antibody (1E1C7) Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]](https://resources.rndsystems.com/images/products/nbp3-57405_-immunohistochemistry-paraffin-639289274469062006.jpg "Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]")
Immunohistochemistry-Paraffin: DLL3 Antibody (1E1C7) [NBP3-57405]
Immunohistochemistry analysis of paraffin-embedded Human small cell lung cancer tissue using NBP3-57405 at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for DLL3 Antibody (1E1C7)
Application
Recommended Usage
ELISA
1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunohistochemistry-Paraffin
1:200 - 1:800
Immunoprecipitation
0.15 ug - 0.3 ug antibody for 200 ug - 400 ug extracts of whole cells
Western Blot
1:2000 - 1:10000
Application Notes
For high-ratio antibody dilutions (greater than 1:10000), a sequential dilution method is strongly recommended to ensure measurement accuracy.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, (pH 7.3)
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DLL3
Long Name
Delta-like 3
Alternate Names
Pudgy, SCDO1
Gene Symbol
DLL3
Additional DLL3 Products
Product Documents for DLL3 Antibody (1E1C7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DLL3 Antibody (1E1C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
