DNA Antibody (AC-30-10)

Novus Biologicals | Catalog # NB110-89473

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bacteria

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # AC-30-10
Loading...

Product Specifications

Immunogen

Human DNA

Reactivity Notes

Predicted cross-reactivities: Insect, Chicken, Amphibia, Mouse, Rat

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Applications for DNA Antibody (AC-30-10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10

Immunohistochemistry-Paraffin

1:10

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS, 0.5% Bovine Serum Albumin

Preservative

0.1% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA

dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Alternate Names

Double stranded deoxyribonucleic acid, Double stranded DNA, dsDNA

Additional DNA Products

Product Documents for DNA Antibody (AC-30-10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DNA Antibody (AC-30-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA Antibody (AC-30-10)

Customer Reviews for DNA Antibody (AC-30-10)

There are currently no reviews for this product. Be the first to review DNA Antibody (AC-30-10) and earn rewards!

Have you used DNA Antibody (AC-30-10)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DNA Antibody (AC-30-10)

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Is the DNA antibody AC-30-10 specific against double-stranded DNA or does it also bind to single-stranded DNA?

    A: According to our datasheet, NB110-89473 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa. It should bind both DNA forms.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies
Loading...