DNA Ligase IV Antibody - BSA Free

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Western Blot: DNA Ligase IV Antibody [NBP2-16182] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (NBP2-16182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: DNA Ligase IV Antibody [NBP2-16182] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (NBP2-16182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: DNA Ligase IV Antibody - BSA Free [NBP2-16182] -

Obesity alters the levels of DSB end-joining repair proteins.a Western blot analysis of NHEJ pathway proteins: Ku70, DNA-PK, XRCC4, Ligase IV, and alternative-NHEJ pathway proteins: MRE11, Rad50, XRCC1, Ligase III (normalized to loading controls beta -actin or Vinculin) from testicular tissue extracts. For comparison, protein lysates from B-DNA mice (CD, N = 5; HFD, N = 5) were run on one blot and H-DNA mice (CD, N = 4; HFD, N = 5) were run on a separate blot. b, c Quantitation of blots represented as violin plots with all data points and dotted lines indicating median and quartile. Statistical analysis was performed using unpaired, two-tailed Mann‒Whitney U test with 95% confidence interval. p > 0.05 (not significant), *p > 0.05, **p > 0.01. Source data are provided as a Source Data file. Statistical analysis is provided as Supplementary Note 2. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-50006-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.