DNA Polymerase beta Antibody (HL1512)
Novus Biologicals | Catalog # NBP3-48656
Recombinant Monoclonal Antibody
Loading...
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1512
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human DNA Polymerase beta. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNA Polymerase beta Antibody (HL1512)
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.
DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 10 umin.
Green: DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.
DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for DNA Polymerase beta Antibody (HL1512)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Polymerase beta
Alternate Names
POLB
Gene Symbol
POLB
Additional DNA Polymerase beta Products
Product Documents for DNA Polymerase beta Antibody (HL1512)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DNA Polymerase beta Antibody (HL1512)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for DNA Polymerase beta Antibody (HL1512)
There are currently no reviews for this product. Be the first to review DNA Polymerase beta Antibody (HL1512) and earn rewards!
Have you used DNA Polymerase beta Antibody (HL1512)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...