DNA Polymerase beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03454

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNA Polymerase beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Polymerase beta Antibody - BSA Free

Western Blot: DNA Polymerase beta AntibodyBSA Free [NBP3-03454]

Western Blot: DNA Polymerase beta AntibodyBSA Free [NBP3-03454]

Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Analysis of extracts of various cell lines, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunocytochemistry/Immunofluorescence: DNA Polymerase beta Antibody [NBP3-03454] - Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (NBP3-03454). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Mouse kidney using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-03454] - Human stomach using DNA Polymerase beta antibody (NBP3-03454) at dilution of 1:100 (40x lens).
Knockout Validated: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Western Blot: DNA Polymerase beta Antibody - BSA Free [NBP3-03454]

Western Blot: DNA Polymerase beta Antibody [NBP3-03454] - Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (NBP3-03454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for DNA Polymerase beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Polymerase beta

DNA polymerase beta comprises an amino terminal 8kDa domain and a carboxy terminal 31kDa domain. DNA Polymerase beta, a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Alternate Names

POLB

Gene Symbol

POLB

Additional DNA Polymerase beta Products

Product Documents for DNA Polymerase beta Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DNA Polymerase beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

Customer Reviews for DNA Polymerase beta Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DNA Polymerase beta Antibody - BSA Free and earn rewards!

Have you used DNA Polymerase beta Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies