DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25609
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2150 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human DNA Polymerase epsilon catalytic subunit A.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] -
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (NBP3-25609) diluted at 1:900. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] -
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (NBP3-25609) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) [NBP3-25609] -
DNA Polymerase epsilon catalytic subunit A antibody [HL2150] detects DNA Polymerase epsilon catalytic subunit A protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.
DNA Polymerase epsilon catalytic subunit A stained by DNA Polymerase epsilon catalytic subunit A antibody [HL2150] (NBP3-25609) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Polymerase epsilon catalytic subunit A
Alternate Names
DKFZp434F222, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, EC 2.7.7, EC 2.7.7.7, POLE1FLJ21434, polymerase (DNA directed), epsilon
Gene Symbol
POLE
Additional DNA Polymerase epsilon catalytic subunit A Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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