DNA Polymerase gamma Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-52300

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNA Polymerase gamma Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA Polymerase gamma.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35121402).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Product can be stored undiluted at 4C for up to 1-2 weeks.

Scientific Data Images for DNA Polymerase gamma Antibody - BSA Free

Western Blot: DNA Polymerase gamma Antibody [NBP1-52300]

Western Blot: DNA Polymerase gamma Antibody [NBP1-52300]

Western Blot: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. POLG antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300]

Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300]

Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with methanol-fixed HeLa cell at 1:200 dilution.
Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300]

Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300]

Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with human skeletal muscle at concentration 10 ug/ml.

Applications for DNA Polymerase gamma Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry

1:100-1:200

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1:1000-1:10000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7), 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

0.67 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: DNA Polymerase gamma

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Long Name

DNA polymerase subunit gamma-1

Alternate Names

MDP1, POLG, PolG-alpha, POLG1, POLGA

Entrez Gene IDs

5428 (Human)

Gene Symbol

POLG

UniProt

P54098 (Human)

Additional DNA Polymerase gamma Products

Product Documents for DNA Polymerase gamma Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA Polymerase gamma Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for DNA Polymerase gamma Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DNA Polymerase gamma Antibody - BSA Free

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  • Q: I am planning on using your antibody NBP1-52300 and am wondering about the immunogen. I understand that you might not be able to give me the exact sequence, but it is of great importance to me that the antibody does not bind to amino acids 994-1040. Is it possible for you to answer whether the antibody binds to this position or not?

    A: The antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma. We typically do not epitope map our antibodies and I cannot determine if this antibody does or does not bind to amino acids 994-1040 however the immunogen sequence does include that fragment.

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