DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

Novus Biologicals | Catalog # H00051426-M01

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Binding Activity

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6F2

Format

Azide and BSA Free
View all DNA Polymerase Kappa Primary Antibodies »

Product Specifications

Immunogen

POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP

Reactivity Notes

Other species not tested.

Specificity

POLK - polymerase (DNA directed) kappa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Immunocytochemistry/ Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.
ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01]

ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.

Applications for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00051426-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA Polymerase Kappa

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity

Alternate Names

DINB protein, DINPPOLQDINB1EC 2.7.7.7, DNA polymerase kappa, polymerase (DNA directed) kappa

Entrez Gene IDs

51426 (Human)

Gene Symbol

POLK

OMIM

605650 (Human)

UniProt

AAH50718 (Human)

Additional DNA Polymerase Kappa Products

Product Documents for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free

Customer Reviews for DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: T98G, A172, U87MG and normal human astrocyte lysates
    Species: Human
    Verified Customer | Posted 01/07/2015
    Immunoblot against hpol kappa (Novus H00051426-M01) on whole cell lysates from A172, T98G, U87MG, and normal human astrocytes.
    DNA Polymerase Kappa Antibody (6F2) - Azide and BSA Free H00051426-M01

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