DNase II Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76971

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNase II Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

~40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNase II Antibody - BSA Free

Immunohistochemistry: DNase II Antibody - BSA Free [NBP1-76971]

Immunohistochemistry: DNase II Antibody - BSA Free [NBP1-76971]

Immunohistochemistry: DNase II Antibody [NBP1-76971] - Staining of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.
DNase II Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DNase II Antibody - BSA Free [NBP1-76971] -

Immunocytochemistry/ Immunofluorescence: DNase II Antibody - BSA Free [NBP1-76971] - Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.

Green: DNase II antibody
Red: Phylloidin staining
Blue: DAPI staining
DNase II Antibody - BSA Free

Immunohistochemistry: DNase II Antibody - BSA Free [NBP1-76971] -

Immunohistochemistry: DNase II Antibody - BSA Free [NBP1-76971] - Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 ug/mL.
DNase II Antibody - BSA Free

Western Blot: DNase II Antibody - BSA Free [NBP1-76971] -

Western Blot: DNase II Antibody - BSA Free [NBP1-76971] - Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

Applications for DNase II Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 ug/ml
Application Notes
An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNase II

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.

Alternate Names

Acid DNase, Deoxyribonuclease II alpha, deoxyribonuclease II, lysosomal, deoxyribonuclease-2-alpha, DNase II alpha, DNase II, lysosomal, DNASE2A, DNL, DNL2, EC 3.1.22.1, Lysosomal DNase II, R31240_2

Entrez Gene IDs

1777 (Human)

Gene Symbol

DNASE2

UniProt

O00115 (Human)

Additional DNase II Products

Product Documents for DNase II Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DNase II Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNase II Antibody - BSA Free

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Protocols

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