Dnmt2 Antibody

Western Blot: Dnmt2 Antibody [NBP3-18663]

Western Blot: Dnmt2 Antibody [NBP3-18663] - Figure 2. Western blot analysis using the antibody directed against Dnmt2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Dnmt2, diluted 1:1000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.

ELISA: Dnmt2 Antibody [NBP3-18663]

ELISA: Dnmt2 Antibody [NBP3-18663] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Dnmt2. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:21,000.