DNMT3B Antibody

Novus Biologicals | Catalog # NB300-516

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DNMT3B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218) Human reactivity reported in scientific literature (PMID: 20847044). Mouse reactivity reported in scientific literature (PMID: 21149390)..

Localization

Nuclear

Specificity

This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DNMT3B Antibody (NB300-516) is a polyclonal antibody validated for use in IHC, WB, Simple Western and ChIP. Anti-DNMT3B Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DNMT3B Antibody

Western Blot: DNMT3B Antibody [NB300-516]

Western Blot: DNMT3B Antibody [NB300-516]

DNMT3B-Antibody-Western-Blot-NB300-516-img0014.jpg
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).
Western Blot: DNMT3B Antibody [NB300-516]

Western Blot: DNMT3B Antibody [NB300-516]

Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.
Western Blot: DNMT3B Antibody [NB300-516]

Western Blot: DNMT3B Antibody [NB300-516]

DNMT3B-Antibody-Western-Blot-NB300-516-img0012.jpg
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516]

Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left).
Simple Western: DNMT3B Antibody [NB300-516]

Simple Western: DNMT3B Antibody [NB300-516]

Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
Simple Western: DNMT3B Antibody [NB300-516]

Simple Western: DNMT3B Antibody [NB300-516]

Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.
Knockdown Validated: DNMT3B Antibody [NB300-516]

Western Blot: DNMT3B Antibody [NB300-516]

DNMT3B-Antibody-Knockdown-Validated-NB300-516-img0013.jpg
DNMT3B Antibody

Chromatin Immunoprecipitation: DNMT3B Antibody [NB300-516] -

Epigenetic changes & protein binding at Ankrd26 promoter in eAT from mice upon 22 weeks of HFD.ChIP of DNMT1, DNMT3a, DNMT3b (a) & MBD2 (b) binding at Ankrd26 promoter region (−553 bp/−348 bp). (c) MNase for Nuc-2 (−257 bp/−198 bp) & Nuc-1 (−84 bp/−25 bp) occupancy at Ankrd26 promoter. (d) ChIP for acetyl-H4 enrichment at Nuc-2 & Nuc-1. (e) ChIP of RNA Pol II binding at Ankrd26 TSS (+16 bp/+159 bp). (a,b) & (d,e), ChIP enrichment is relative to Input chromatin. (a–e), results are mean ± SD from three independent experiments. **p < 0.01 & ***p < 0.001 vs STD. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep43526), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DNMT3B Antibody

Western Blot: DNMT3B Antibody [NB300-516] -

Western Blot: DNMT3B Antibody [NB300-516] - DNMT3B is a direct target of miR-30a & miR-379. a The effect of miRNA mimics (PM-30a or PM-379, 20 nM) on the luciferase activities of the constructs containing the wild-type (wt-3′-UTR) or mutant-type 3′-UTR (mt-30a-3′-UTR or mt-379-3′-UTR) in OEC-M1 cells. The relative luciferase activity of each sample is measured at 48 h after transfection & normalized to Renilla luciferase activity. The data are represented as mean ± SD; ***p < 0.001 versus control mimics (PM-NC). b RT-PCR & Western blot analysis of DNMT3B level in SCC-15 & OEC-M1 cells after treatment with control mimics (NC), or miRNA mimics (PM-30a or PM-379, 20 nM) or miRNA inhibitor (AM-30a or AM-379, 20 nM) for 48 h. GAPDH & alpha -tubulin were used as internal control, respectively. c RT-PCR analysis of ADHFE1 & ALDH1A2 expression level after treatment with control mimics (NC, 20 nM), or miRNA mimics (PM-30a or PM-379, 20 nM) for 48 h in SCC-15 & OEC-M1 cells. GAPDH was used as internal control. d Relative miR-30a & miR-379 expression levels in 33 of adjacent normal tissues (N) compared with their own tumors (T). e Correlation analysis of DNMT3B & miR-30a or miR-379 in OSCC patients (n = 33) by qRT-PCR analysis. Pearson correlation coefficients & p-values were calculated as indicated. Red, tumor part; green, normal part Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DNMT3B Antibody

Western Blot: DNMT3B Antibody [NB300-516] -

Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DNMT3B Antibody

Western Blot: DNMT3B Antibody [NB300-516] -

Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DNMT3B Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:1000

Simple Western

20 ug/mL

Western Blot

2.0 ug/mL
Application Notes

May be Useful in WB. ChIP usage was reported in scientific literature. WB: Detects a 130 kDa band is seen in P19 nuclear extracts.
See Simple Western Antibody Database for Simple Western validation: Tested in BG01V lysate 0.5 mg/mL, separated by Size, antibody dilution of 20 ug/mL

Reviewed Applications

Read 3 reviews rated 3.7 using NB300-516 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNMT3B

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.

Long Name

DNA [Cytosine-5-]-Methyltransferase 3B

Alternate Names

ICF1, M.HsaIIIB

Entrez Gene IDs

1789 (Human); 13436 (Mouse)

Gene Symbol

DNMT3B

UniProt

O88509 (Mouse)

Additional DNMT3B Products

Product Documents for DNMT3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DNMT3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

Citations for DNMT3B Antibody

Customer Reviews for DNMT3B Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
33%

Have you used DNMT3B Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • DNMT3B Antibody
    Name: Jeremie Raso
    Application: Simple Western
    Sample Tested: HepG2 CELLS
    Species: Human
    Verified Customer | Posted 02/03/2020
    materiel: 1x25 caps 12-230kDa échantillon : 0.08 à 1µg/µL anticorps : 1/250 à 1/10
    DNMT3B Antibody NB300-516
  • DNMT3B Antibody
    Name: Yichi Xu
    Application: Chromatin Immunoprecipitation
    Sample Tested: cell
    Species: Human
    Verified Customer | Posted 01/30/2019
    DNMT3B Antibody NB300-516
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Lysate from homogenized mouse brain cells
    Species: Mouse
    Verified Customer | Posted 07/17/2014
    WB for Dnmt3b in mouse cells
    DNMT3B Antibody NB300-516

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies