DOG1/TMEM16A Antibody (SPM580)

Novus Biologicals | Catalog # NBP2-32841

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM580
View all ANO1/TMEM16A Primary Antibodies »

Product Specifications

Immunogen

Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)

Localization

Cell Surface and Cytoplasm

Marker

Marker for Gastrointestinal Stromal Tumors

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34812)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for DOG1/TMEM16A Antibody (SPM580)

Immunocytochemistry/ Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841]

Immunocytochemistry/ Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841]

Immunocytochemistry/Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841] - HEK cells expressing Ano1-Myc. SPM580 gave more signal than the Myc Ab control and much more than the SP31 mAb. Negative control cells were negative. ICC image submitted by a verified customer review.
Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841] - Formalin-fixed paraffin-embedded human GIST stained with DOG1 Monoclonal Antibody (SPM580).

Applications for DOG1/TMEM16A Antibody (SPM580)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Validated for ICC/IF & WB from a verified customer review.

Reviewed Applications

Read 2 reviews rated 3 using NBP2-32841 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ANO1/TMEM16A

Long Name

Anoctamin 1

Alternate Names

DOG1, ORAOV2, TAOS2, TMEM16A

Gene Symbol

ANO1

UniProt

Q5XXA6 (Human)

Additional ANO1/TMEM16A Products

Product Documents for DOG1/TMEM16A Antibody (SPM580)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DOG1/TMEM16A Antibody (SPM580)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DOG1/TMEM16A Antibody (SPM580) (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Customer Images

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  • DOG1/TMEM16A Antibody (SPM580)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: protein lysates and MDCK kidney protein lysate
    Species: MDCK
    Verified Customer | Posted 11/27/2019
    50µg Protein load on normal SDS gel 20 min at 70V and then 1h at 120V Tank blotting for 3h at 120V in PVDF membrane Blocking for 1h with 5%MM/TBST Prim. AB 1:500 in 1%MM/TBST over night
    DOG1/TMEM16A Antibody (SPM580) NBP2-32841
  • DOG1/TMEM16A Antibody (SPM580)
    Name: Randolph Elble
    Application: Immunocytochemistry
    Sample Tested: Hek 293T
    Species: Human
    Verified Customer | Posted 02/01/2017
    HEK cells expressing Ano1-Myc. SPM580 gave more signal than the Myc Ab control and Much more than the SP31 mAb. Negative control cells were negative. The Santa Cruz mAb and pAb for Ano1 were negative on these cells.
    DOG1/TMEM16A Antibody (SPM580) NBP2-32841

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Protocols

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