DOK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92597

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DOK4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human DOK4 (NP_060580.2). MATNFSDIVKQGYVKMKSRKLGIYRRCWLVFRKSSSKGPQRLEKYPDEKSVCLRGCPKVTEISNVKCVTRLPKETKRQAVAIIFTDDSARTFTCDSELEAEEWYKTLSVECLGSRLNDISLGEPDLLAPGVQCEQTDRFNVFLLPCPNLDVYGECKLQITHENIYLWDIHNPRVKLVSWPLCSLRRYGRDATRFTFEAGRMCDAGEGLYTFQTQEGEQIYQRVHSATLAIAEQHKRVLLEMEKNVRLLNKGTEHYSYPCTPTTMLPRSAYWHHITGSQNIAEASSYAGEGYGAAQASSETDLLNRFILLKPKPSQGDSSEAKTPSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOK4 Antibody - BSA Free

Western Blot: DOK4 AntibodyAzide and BSA Free [NBP2-92597]

Western Blot: DOK4 AntibodyAzide and BSA Free [NBP2-92597]

Western Blot: DOK4 Antibody [NBP2-92597] - Western blot analysis of extracts of various cell lines, using DOK4 antibody (NBP2-92597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: DOK4 Antibody - Azide and BSA Free [NBP2-92597]

Immunohistochemistry-Paraffin: DOK4 Antibody - Azide and BSA Free [NBP2-92597]

Immunohistochemistry-Paraffin: DOK4 Antibody [NBP2-92597] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (NBP2-92597) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
DOK4 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] -

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of PC-12 cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
DOK4 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] -

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of NIH/3T3 cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
DOK4 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] -

Immunocytochemistry/ Immunofluorescence: DOK4 Antibody - Azide and BSA Free [NBP2-92597] - Immunofluorescence analysis of SH-SY5Y cells using DOK4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for DOK4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOK4

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells

Long Name

Docking Protein 4

Alternate Names

docking protein 4, Downstream of tyrosine kinase 4, FLJ10488, Insulin receptor substrate 5, IRS5, IRS-5

Gene Symbol

DOK4

Additional DOK4 Products

Product Documents for DOK4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DOK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

GDNF Family

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Protocols

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FAQs

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