Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25442

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1163 expressed in HEK293

Format

Azide and BSA Free
View all Dopamine beta-Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dopamine beta-Hydroxylase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%), Rhesus Macaque (86%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -

Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.
Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -

Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -

Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine beta-Hydroxylase

Dopamine beta-hydroxylase belongs to the copper type II, ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to noradrenaline. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons being expressed in noradrenergic and adrenergic neurons. DBH serves as a marker of noradrenergic cells.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Gene Symbol

DBH

Additional Dopamine beta-Hydroxylase Products

Product Documents for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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