Dopamine D1R/DRD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16213

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Dopamine D1R/DRD1. The exact sequence is proprietary.

Localization

Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine D1R/DRD1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213]

Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213]

Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213] - U373 xenograft, using Dopamine Receptor D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Dopamine D1R/DRD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Dopamine D1R/DRD1 Antibody [NBP2-16213] -

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Dopamine D1R/DRD1 Antibody - BSA Free

Immunohistochemistry-Frozen: Dopamine D1R/DRD1 Antibody [NBP2-16213] -

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:250.
Blue: Fluoroshield with DAPI.
Dopamine D1R/DRD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Dopamine D1R/DRD1 Antibody [NBP2-16213] -

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Dopamine D1R/DRD1 Antibody - BSA Free

Western Blot: Dopamine D1R/DRD1 Antibody - BSA Free [NBP2-16213] -

ICAM-1 reduces neurotransmitters expression that reflects in sensorimotor deficits and psychological stress after TBI. A, Immunofluorescent staining of NE (red) in the hippocampus area of WT and ICAM-1−/− mice after 10 and 20 psi FPI and merged with NeuN (green) and DAPI (blue). Scale bar: 20 μm (all panels). B, Quantification of NE staining in the hippocampus area of uninjured, 10 and 20 psi FPI WT and ICAM-1−/− mice using ImageJ software (n = 6/group). C–E, Western blot analysis of 5-HT1AR (C), DAD1R (D), NPY (E) and beta -actin in the tissue lysates of hippocampus of WT and ICAM-1−/− mice 48 h after 10 and 20 psi FPI. The bar graph with dot plots shows the quantification of 5-HT1AR (C), DAD1R (D), NPY (E) versus beta -actin (n = 6/group). F, Schematic presentation of the findings. All values are expressed as mean +/- SD two-way ANOVA followed by Bonferroni post hoc tests. Statistically significant ***p < 0.001 versus WT uninjured group; @@@p < 0.001 versus uninjured ICAM-1−/− group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus WT corresponding injury groups; ns = non-significant. NE, norepinephrine; 5-HT1AR, 5-HT 1A receptor; DAD1R, DA D1 receptor; NPY, neuropeptide Y. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34135004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Dopamine D1R/DRD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-16213 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dopamine D1R/DRD1

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Long Name

Dopamine D1 Receptor

Alternate Names

DADR, Dopamine D1 R, DRD1

Gene Symbol

DRD1

UniProt

Additional Dopamine D1R/DRD1 Products

Product Documents for Dopamine D1R/DRD1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Dopamine D1R/DRD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Dopamine D1R/DRD1 Antibody - BSA Free

Customer Reviews for Dopamine D1R/DRD1 Antibody - BSA Free (1)

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  • Dopamine D1 R/DRD1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: 5 um Mouse brain paraffin slides and 5 um human bone paraffin slides
    Species: human bone tissue and mouse brain
    Verified Customer | Posted 04/18/2017

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