DOT1L Antibody

Western Blot: DOT1L Antibody [NB100-40845]

Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody NB100-40845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).

Western Blot: DOT1L Antibody [NB100-40845]

DOT1L-Antibody-Western-Blot-NB100-40845-img0015.jpg

Western Blot: DOT1L Antibody [NB100-40845] -

siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression (a) and a decrease of DOT1L protein level (b). Empty bars are untreated cells and solid bars are transfected cells. Results from HSkMSC cultures derived from three different experiments on cells at passage three, Graph is presenting means ± SEM. *, p-value < 0.05 siRNA treated cells compared to controls