DRP1 Antibody (3B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00010059-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B5

Format

Azide and BSA Free
View all DRP1 Primary Antibodies »

Product Specifications

Immunogen

DNM1L (AAH24590.1, 1 a.a. ~ 710 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29092894). Other species not tested.

Specificity

DNM1L - dynamin 1-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for DRP1 Antibody (3B5) - Azide and BSA Free

Western Blot: DRP1 Antibody (3B5) [H00010059-M01]

Western Blot: DRP1 Antibody (3B5) [H00010059-M01]

Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.
Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01]

Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01]

Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. Antibody concentration 1 ug/ml.
Western Blot: DRP1 Antibody (3B5) [H00010059-M01]

Western Blot: DRP1 Antibody (3B5) [H00010059-M01]

Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody (M01), clone 3B5.Lane 1: DNM1L transfected lysate(79 KDa).Lane 2: Non-transfected lysate.
ELISA: DRP1 Antibody (3B5) [H00010059-M01]

ELISA: DRP1 Antibody (3B5) [H00010059-M01]

ELISA: DRP1 Antibody (3B5) [H00010059-M01] - Detection limit for recombinant GST tagged DNM1L is approximately 0.3ng/ml as a capture antibody.

Applications for DRP1 Antibody (3B5) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DRP1

The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated.

Long Name

Dynamin-1-like protein

Alternate Names

DLP1, DNM1L, DVLP, Dymple, HdynIV

Entrez Gene IDs

10059 (Human)

Gene Symbol

DNM1L

OMIM

603850 (Human)

UniProt

AAH24590 (Human)

Additional DRP1 Products

Product Documents for DRP1 Antibody (3B5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DRP1 Antibody (3B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DRP1 Antibody (3B5) - Azide and BSA Free

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Protocols

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FAQs

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