Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Fish, Primate

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all DRP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Fish reactivity reported in scientific literature (PMID: 25008790).

Localization

Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DRP1 Antibody [FITC]

DRP1 Antibody [FITC]

Detection of DRP1 in SJCRH30 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on SJCRH30 human Rhabdomyosarcoma cell line with Rabbit anti-DRP1 Affinity-purified Polyclonal Antibody conjugated to FITC (Catalog # NB110-55288F, blue histogram) or matched control antibody (NBP2-24892, orange histogram) at 5 µg/mL for 30 minutes at RT.

Applications for DRP1 Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DRP1

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Long Name

Dynamin-1-like protein

Alternate Names

DLP1, DNM1L, DVLP, Dymple, HdynIV

Gene Symbol

DNM1L

Additional DRP1 Products

Product Documents for DRP1 Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for DRP1 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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