Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # J5

Format

Azide and BSA Free
View all dsRNA Primary Antibodies »

Product Specifications

Immunogen

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Specificity

This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by J5, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. The antibody does not react with ssRNA, ssDNA or dsDNA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

After reconstitution antibodies should be aliquoted and stored at -20C or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for at least 5 years at -20C or -70C. This ds-RNA antibody was manufactured by Scicon.

Applications for dsRNA Antibody (J5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of the antibody for any specific application should be established by titration.

Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.

Not for use for clinical purposes. For in vitro use only.

dsRNA-immunoblotting.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml.

Formulation

Lyophilised from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: dsRNA

Double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. The J5 antibody can detect all tested forms of dsRNA, including poly(A):poly(U), poly(I):poly(C) and dsRNA from viruses such as Dengue Virus, Encephalomyocarditis Virus, Vaccinia Virus, Reovirus or Cucumber Mosaic Virus.

Alternate Names

Double-stranded RNA

Additional dsRNA Products

Product Documents for dsRNA Antibody (J5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for dsRNA Antibody (J5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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