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R&D Systems Dual-Color ELISpot Kits are complete microplate-based immunoassays designed for the detection and enumeration of two different immune cell populations in a single sample. ELISpot assays are highly sensitive and can quantitate actively secreting cells even when cell frequencies fall below 1 in 100,000. Unlike other methods that assess antigen-specific immune cell responses, this assay does not require prior in vitro expansion of cells and is suitable for high-throughput analysis. These Dual-Color ELISpot Kits are complete ready-to-run assays and require no further development or refinement.
R&D Systems Dual-Color ELISpot Kits employ antibody-coated, membrane-backed microplates to identify single cells that secrete either one or both analytes specified in the kit. The immobilized capture antibodies bind to cytokines that have been secreted in their vicinity, and enzyme conjugates are used to visualize the secreted proteins. Colored precipitates form at the sites of cytokine localization and appear as spots, with each individual spot representing an individual cytokine-secreting cell.
|IFN-gamma (blue spots) and IL-17 (red spots) were secreted from mouse splenocytes stimulated with PMA/Ca2+ ionomycin. Spots of cytokine secretion were visualized using the Mouse IFN-gamma/IL-17 Dual-Color ELISpot Kit (Catalog # ELD5007).|