DuoSet ELISA Ancillary Reagent Kit 2

Catalog # Availability Size / Price Qty
DY008
R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (36)

DuoSet ELISA Ancillary Reagent Kit 2 Summary

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.


PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
  • Wash Buffer (25X, six 21 mL vials)

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for DuoSet ELISA Ancillary Reagent Kit 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Potential relation between soluble growth differentiation factor-15 and testosterone deficiency in male patients with coronary artery disease
    Authors: H Liu, Y Lyu, D Li, Y Cui, Y Huang, W Dai, Y Li
    Cardiovasc Diabetol, 2019;18(1):21.  2019
  2. Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
    Authors: S Pereira, SM O'Dwyer, TD Webber, RK Baker, V So, CE Ellis, JS Yoon, M Mojibian, MM Glavas, S Karunakara, SM Clee, SD Covey, TJ Kieffer
    Sci Rep, 2019;9(1):3307.  2019
  3. Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children
    Authors: A Posod, R Pechlaner, X Yin, SA Burnap, SJ Kiechl, J Willeit, JL Witztum, M Mayr, S Kiechl, U Kiechl-Koh
    J Am Heart Assoc, 2019;8(8):e011199.  2019
  4. CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
    Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
    J. Cell. Mol. Med., 2018;0(0):.  2018
  5. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
    Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
    Nat Commun, 2018;9(1):1988.  2018
  6. Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
    Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
    Cell Rep, 2018;25(3):677-689.e4.  2018
  7. Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
    Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
    Int J Mol Sci, 2018;19(12):.  2018
  8. Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
    Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
    J. Biol. Chem., 2017;0(0):.  2017
  9. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.  2017
  10. Urinary kallikrein 10 predicts the incurability of gastric cancer
    Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
    Oncotarget, 2017;8(17):29247-29257.  2017
  11. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017;7(1):6871.  2017
  12. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017;7(4):e1079.  2017
  13. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
    Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
    Mediators Inflamm., 2017;2017(0):9734837.  2017
  14. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.  2017
  15. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016;0(0):.  2016
  16. Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
    Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
    J Immunol, 0;200(8):2581-2591.  0

FAQs

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Reviews for DuoSet ELISA Ancillary Reagent Kit 2

Average Rating: 4.8 (Based on 36 Reviews)

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4 Star
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 07/02/2019

Contains all the reagents needed and works well, p.s. nice ladder


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/30/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/23/2019

The perfect companion to DuoSet ELISA kits, everything you need to run five plates.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/19/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/11/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/31/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/09/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/11/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/08/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 07/17/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/21/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/20/2018

I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/19/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/04/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/26/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/30/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/01/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/01/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/20/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/03/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/16/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/30/2017

Used for measuring NGF in bovine seminal plasma.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/19/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/12/2017

The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/25/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/12/2017

DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/26/2017

We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/20/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/06/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/01/2016

Every reagent provided.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2016

This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.

The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.

The box it is provided in is more ergonomic now than its previous versions as well.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/03/2016

This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/19/2016

Works very well with all compatible DuoSet kits.


DuoSet ELISA Ancillary Reagent Kit 2, 5 Plate
By Anonymous on 01/08/2016