DuoSet ELISA Ancillary Reagent Kit 2
R&D Systems | Catalog # DY008
Product in process of being discontinued and replaced by new Next generation kit
Discontinued Product
DY008 has been discontinued.
An alternative/replacement product is available:
DY008B. View all Buffers, Chelators and Reagents products.
Key Product Details
Applications
ELISA
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Product Summary for DuoSet ELISA Ancillary Reagent Kit 2
Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
PRODUCT FEATURES
- Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
KIT CONTENTS
- Clear Polystyrene Microplates (five 12x8-well strip microplates)
- ELISA Plate Sealers (24 plate sealers)
- Color Reagent A (1X, three 12.5 mL vials)
- Color Reagent B (1X, three 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
- Wash Buffer (25X, six 21 mL vials)
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Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Product Documents for DuoSet ELISA Ancillary Reagent Kit 2
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for DuoSet ELISA Ancillary Reagent Kit 2
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for DuoSet ELISA Ancillary Reagent Kit 2
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Customer Reviews for DuoSet ELISA Ancillary Reagent Kit 2 (93)
4.9 out of 5
93 Customer Ratings
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Verified Customer | Posted 09/30/2024
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Verified Customer | Posted 04/24/2023
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Verified Customer | Posted 02/07/2023
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Verified Customer | Posted 11/21/2022HepG2 conditioned medium
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Verified Customer | Posted 11/02/2022
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Verified Customer | Posted 11/02/2022LOW BLANK SIGNAL. HENCE EXCELLENT.
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Verified Customer | Posted 06/18/2022
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Verified Customer | Posted 05/31/2022
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Verified Customer | Posted 05/26/2022
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Verified Customer | Posted 03/24/2022Works very well if protocol is followed. Another high binding plates can also be used optionally
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Verified Customer | Posted 03/16/2022Budget friendly and produces excellent results
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Verified Customer | Posted 03/11/2022Used with Mouse CXCL10 ELISA Kit DY446
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Verified Customer | Posted 12/16/2021
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Verified Customer | Posted 12/11/2021
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Verified Customer | Posted 12/09/2021
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Verified Customer | Posted 10/06/2021
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Verified Customer | Posted 09/28/2021
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Verified Customer | Posted 09/23/2021
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Verified Customer | Posted 08/19/2021
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Verified Customer | Posted 08/06/2021Very good kit. It’s reasonable to buy this kit instead of buying everything separately. The result is reproducible.
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Verified Customer | Posted 08/06/2021This kit is very helpful. The protocol is also easy and straight forward
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Verified Customer | Posted 05/18/2021super convenient and budge friendly
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Verified Customer | Posted 04/09/2021Results are very reproducible and the kit was very helpful and easy to use. This saved a lot of time, with minimal troubleshooting.
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Verified Customer | Posted 04/09/2021
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Verified Customer | Posted 12/20/2020The results are reproducible between different Lots of this kit.
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Verified Customer | Posted 12/20/2020Results are reproducible and the kit is easy to follow.
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Verified Customer | Posted 12/18/2020Used for measuring IL2 and IFN-gamma release in T cell cultures.
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Verified Customer | Posted 12/01/2020
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Verified Customer | Posted 11/28/2020The DuoSet ELISA Ancillary Reagent Kit 2 was used with the Human TNF-alpha DuoSet ELISA kit (DY210), Human IL-6 DuoSet ELISA (DY206) and Human IL-12/IL-23 p40 DuoSet ELISA (DY124) to detect secreted cytokines in GMCSF/IL4 monocyte-derived human dendritic cells stimulated with LPS (100 ng/ml) for 24 hours.
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Verified Customer | Posted 11/06/2020
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Verified Customer | Posted 10/28/2020
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Verified Customer | Posted 10/27/2020I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.
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Verified Customer | Posted 10/23/2020Works well!!!
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Verified Customer | Posted 10/23/2020
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Verified Customer | Posted 10/13/2020The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.
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Verified Customer | Posted 10/13/2020DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.
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Verified Customer | Posted 10/07/2020
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Verified Customer | Posted 09/30/2020Used for quantifying release of IL2 and IFN gamma
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Verified Customer | Posted 09/29/2020Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.
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Verified Customer | Posted 09/14/2020Fantastic kit. Very cost effective compared to buying everything separately. Reagent Diluent can be scarce depending on sample protein concentration.
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Verified Customer | Posted 08/05/2020
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Verified Customer | Posted 06/08/2020
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Verified Customer | Posted 03/17/2020VERY SENSITIVE, GOOD KIT
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Verified Customer | Posted 01/23/2020Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment. On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.
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Verified Customer | Posted 01/14/2020
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Verified Customer | Posted 01/14/2020
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Verified Customer | Posted 01/11/2020
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Verified Customer | Posted 11/14/2019Contains all the reagents needed for the experiments and works well!Well done!
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Verified Customer | Posted 10/29/2019
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Verified Customer | Posted 10/29/2019
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Verified Customer | Posted 10/29/2019Good standard curve even for linear form
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Verified Customer | Posted 10/06/2019The kit works very well.
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Verified Customer | Posted 10/02/2019
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Verified Customer | Posted 09/23/2019we used this kit for developing sandwich ELISA's
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Verified Customer | Posted 08/21/2019
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Verified Customer | Posted 08/13/2019
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Verified Customer | Posted 08/08/2019
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Verified Customer | Posted 07/02/2019Contains all the reagents needed and works well, p.s. nice ladder
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Verified Customer | Posted 03/30/2019
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Verified Customer | Posted 03/23/2019The perfect companion to DuoSet ELISA kits, everything you need to run five plates.
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Verified Customer | Posted 03/19/2019
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Verified Customer | Posted 01/14/2019
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Verified Customer | Posted 12/11/2018
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Verified Customer | Posted 10/31/2018
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Verified Customer | Posted 10/17/2018
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Verified Customer | Posted 10/09/2018
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Verified Customer | Posted 09/11/2018
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Verified Customer | Posted 08/08/2018
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Verified Customer | Posted 07/17/2018
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Verified Customer | Posted 06/21/2018
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Verified Customer | Posted 06/20/2018I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
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Verified Customer | Posted 06/19/2018
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Verified Customer | Posted 05/04/2018
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Verified Customer | Posted 04/26/2018
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Verified Customer | Posted 03/30/2018
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Verified Customer | Posted 12/01/2017
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Verified Customer | Posted 12/01/2017
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Verified Customer | Posted 10/20/2017
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Verified Customer | Posted 08/03/2017
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Verified Customer | Posted 06/16/2017
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Verified Customer | Posted 05/30/2017Used for measuring NGF in bovine seminal plasma.
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Verified Customer | Posted 05/19/2017
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Verified Customer | Posted 05/12/2017The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
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Verified Customer | Posted 04/25/2017
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Verified Customer | Posted 04/12/2017DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
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Verified Customer | Posted 01/26/2017We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
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Verified Customer | Posted 01/20/2017
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Verified Customer | Posted 01/06/2017
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Verified Customer | Posted 11/01/2016Every reagent provided.
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Verified Customer | Posted 10/17/2016This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit. The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems. The box it is provided in is more ergonomic now than its previous versions as well.
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Verified Customer | Posted 06/03/2016This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
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Verified Customer | Posted 05/19/2016Works very well with all compatible DuoSet kits.
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Verified Customer | Posted 01/08/2016
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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