DuoSet ELISA Ancillary Reagent Kit 2

Catalog # Availability Size / Price Qty
DY008
R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (26)
FAQs
Supplemental Products
Reviews (64)

DuoSet ELISA Ancillary Reagent Kit 2 Summary

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.


PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
  • Wash Buffer (25X, six 21 mL vials)

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

Data Example

R&D Systems DuoSet Ancillary Reagent Kits

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Reagents Provided

Citations for DuoSet ELISA Ancillary Reagent Kit 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors
    Authors: Z Fan, Y Tian, Z Chen, L Liu, Q Zhou, J He, J Coleman, C Dong, N Li, J Huang, C Xu, Z Zhang, S Gao, P Zhou, K Ding, L Chen
    EMBO Mol Med, 2020;0(0):e11571.  2020
  2. Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training
    Authors: J Kortas, E Ziemann, D Juszczak, K Micielska, M Koz?owska, K Prusik, K Prusik, J Antosiewic
    Nutrients, 2020;12(4):.  2020
  3. Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia
    Authors: C Wu, J Lu, S Lu, M Huang, Y Xu
    J Psychiatr Res, 2020;126(0):92-97.  2020
  4. Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes
    Authors: JAD Van, S Clotet-Fre, AC Hauschild, I Batruch, I Jurisica, Y Elia, FH Mahmud, E Sochett, EP Diamandis, JW Scholey, A Konvalinka
    PLoS ONE, 2020;15(5):e0233639.  2020
  5. Potential relation between soluble growth differentiation factor-15 and testosterone deficiency in male patients with coronary artery disease
    Authors: H Liu, Y Lyu, D Li, Y Cui, Y Huang, W Dai, Y Li
    Cardiovasc Diabetol, 2019;18(1):21.  2019
  6. Resistance to autosomal dominant Alzheimer's disease in an APOE3 Christchurch homozygote: a case report
    Authors: JF Arboleda-V, F Lopera, M O'Hare, S Delgado-Ti, C Marino, N Chmielewsk, KL Saez-Torre, D Amarnani, AP Schultz, RA Sperling, D Leyton-Cif, K Chen, A Baena, D Aguillon, S Rios-Romen, M Giraldo, E Guzmán-Vél, DJ Norton, E Pardilla-D, A Artola, JS Sanchez, J Acosta-Uri, M Lalli, KS Kosik, MJ Huentelman, H Zetterberg, K Blennow, RA Reiman, J Luo, Y Chen, P Thiyyagura, Y Su, GR Jun, M Naymik, X Gai, M Bootwalla, J Ji, L Shen, JB Miller, LA Kim, PN Tariot, KA Johnson, EM Reiman, YT Quiroz
    Nat. Med., 2019;0(0):.  2019
  7. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
    Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
    Cell Stem Cell, 2019;0(0):.  2019
  8. A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
    Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
    J Clin Med, 2019;8(10):.  2019
  9. Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
    Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
    Front Neurosci, 2019;13(0):836.  2019
  10. Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children
    Authors: A Posod, R Pechlaner, X Yin, SA Burnap, SJ Kiechl, J Willeit, JL Witztum, M Mayr, S Kiechl, U Kiechl-Koh
    J Am Heart Assoc, 2019;8(8):e011199.  2019
  11. Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
    Authors: S Pereira, SM O'Dwyer, TD Webber, RK Baker, V So, CE Ellis, JS Yoon, M Mojibian, MM Glavas, S Karunakara, SM Clee, SD Covey, TJ Kieffer
    Sci Rep, 2019;9(1):3307.  2019
  12. Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
    Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
    Cell Rep, 2018;25(3):677-689.e4.  2018
  13. Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
    Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
    Int J Mol Sci, 2018;19(12):.  2018
  14. CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
    Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
    J. Cell. Mol. Med., 2018;0(0):.  2018
  15. Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
    Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
    Thyroid, 2018;0(0):.  2018
  16. Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
    Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
    Front Immunol, 2018;9(0):742.  2018
  17. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
    Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
    Nat Commun, 2018;9(1):1988.  2018
  18. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.  2017
  19. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.  2017
  20. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
    Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
    Mediators Inflamm., 2017;2017(0):9734837.  2017
  21. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017;7(4):e1079.  2017
  22. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017;7(1):6871.  2017
  23. Urinary kallikrein 10 predicts the incurability of gastric cancer
    Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
    Oncotarget, 2017;8(17):29247-29257.  2017
  24. Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
    Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
    J. Biol. Chem., 2017;0(0):.  2017
  25. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016;0(0):.  2016
  26. Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
    Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
    J Immunol, 0;200(8):2581-2591.  0

FAQs

  1. Can I use distilled water for diluting the Reagent Diluent Concentrate 2?

    • R&D systems uses deionized water to dilute Reagent Diluent Concentrate 2. We do not have information on how distilled water may affect the performance.

View all ELISA FAQs

Reviews for DuoSet ELISA Ancillary Reagent Kit 2

Average Rating: 4.8 (Based on 64 Reviews)

5 Star
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4 Star
17.19%
3 Star
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/06/2020
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/28/2020
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/27/2020
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I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/23/2020
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Works well!!!


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/23/2020
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/13/2020
Application:

The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/13/2020
Application:

DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/07/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/30/2020
Application:

Used for quantifying release of IL2 and IFN gamma


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/29/2020
Application:

Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/14/2020
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Fantastic kit. Very cost effective compared to buying everything separately. Reagent Diluent can be scarce depending on sample protein concentration.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/05/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/08/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/17/2020
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VERY SENSITIVE, GOOD KIT


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/23/2020
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Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.

On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/11/2020
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/14/2019
Application:

Contains all the reagents needed for the experiments and works well!Well done!


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019
Application:

Good standard curve even for linear form


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/06/2019
Application:

The kit works very well.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/02/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/23/2019
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we used this kit for developing sandwich ELISA's


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/21/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/13/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/08/2019
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 07/02/2019
Application:

Contains all the reagents needed and works well, p.s. nice ladder


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/30/2019
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/23/2019
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The perfect companion to DuoSet ELISA kits, everything you need to run five plates.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/19/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2019
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/11/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/31/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/09/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/11/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/08/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 07/17/2018
Application:

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/21/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/20/2018
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I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/19/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/04/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/26/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/30/2018
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/01/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/01/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/20/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/03/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/16/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/30/2017
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Used for measuring NGF in bovine seminal plasma.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/19/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/12/2017
Application:

The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/25/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/12/2017
Application:

DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/26/2017
Application:

We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/20/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/06/2017
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DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/01/2016
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Every reagent provided.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2016
Application:

This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.

The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.

The box it is provided in is more ergonomic now than its previous versions as well.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/03/2016
Application:

This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/19/2016
Application:

Works very well with all compatible DuoSet kits.


DuoSet ELISA Ancillary Reagent Kit 2, 5 Plate
By Anonymous on 01/08/2016
Application: