DuoSet ELISA Ancillary Reagent Kit 2

  (10 citations)
(26 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (10)
FAQs
Reviews
Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.


PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
  • Wash Buffer (25X, six 21 mL vials)


Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Sample Type
  1. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
    Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
    Nat Commun, 2018;9(1):1988.  2018
  2. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.  2017
  3. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017;7(1):6871.  2017
  4. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.  2017
  5. Urinary kallikrein 10 predicts the incurability of gastric cancer
    Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
    Oncotarget, 2017;8(17):29247-29257.  2017
  6. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
    Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
    Mediators Inflamm., 2017;2017(0):9734837.  2017
  7. Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
    Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
    J. Biol. Chem., 2017;0(0):.  2017
  8. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017;7(4):e1079.  2017
  9. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016;0(0):.  2016
  10. Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
    Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
    J Immunol, 0;200(8):2581-2591.  0

FAQs

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Average Rating: 4.8 (Based on 26 reviews)

Have you used DuoSet ELISA Ancillary Reagent Kit 2?

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Read what people are saying who have used DuoSet ELISA Ancillary Reagent Kit 2.

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Excellent
  Anonymous 08/08/2018
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 07/17/2018
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].
Very Good
  Anonymous 06/21/2018
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 06/20/2018
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].

Other Experimental Details

Other Experimental Details I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
Very Good
  Anonymous 06/19/2018
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Excellent
  Anonymous 05/04/2018
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Excellent
  Anonymous 04/26/2018
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Excellent
  Anonymous 03/30/2018
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 12/01/2017
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].
Excellent
  Anonymous 12/01/2017
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Very Good
  Anonymous 10/20/2017
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Excellent
  Anonymous 08/03/2017
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Excellent
  Anonymous 06/16/2017
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Excellent
  Anonymous 05/30/2017
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Other Experimental Details

Other Experimental Details Used for measuring NGF in bovine seminal plasma.
Excellent
  Anonymous 05/19/2017
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 05/12/2017
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].

Other Experimental Details

Other Experimental Details The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
Very Good
  Anonymous 04/25/2017
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 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 04/12/2017
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].

Other Experimental Details

Other Experimental Details DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
Excellent
  Anonymous 01/26/2017
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Other Experimental Details

Other Experimental Details We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
 DuoSet ELISA Ancillary Reagent Kit 2 DY008 Array DY008
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Excellent
  Anonymous 01/20/2017
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].
Excellent
  Anonymous 01/06/2017
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].
Very Good
  Anonymous 11/01/2016
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Other Experimental Details

Other Experimental Details Every reagent provided.
Very Good
  Anonymous 10/17/2016
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Image Details
 DuoSet ELISA Ancillary Reagent Kit 2 DY008
: DuoSet ELISA Ancillary Reagent Kit 2 [DY008].

Other Experimental Details

Other Experimental Details This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.The box it is provided in is more ergonomic now than its previous versions as well.
Excellent
  Anonymous 06/03/2016
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Other Experimental Details

Other Experimental Details This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
Excellent
  Anonymous 05/19/2016
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Other Experimental Details

Other Experimental Details Works very well with all compatible DuoSet kits.
Excellent
  Anonymous 01/08/2016
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