DuoSet ELISA Ancillary Reagent Kit 2 Summary
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
- Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
- Clear Polystyrene Microplates (five 12x8-well strip microplates)
- ELISA Plate Sealers (24 plate sealers)
- Color Reagent A (1X, three 12.5 mL vials)
- Color Reagent B (1X, three 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
- Wash Buffer (25X, six 21 mL vials)
Citations for DuoSet ELISA Ancillary Reagent Kit 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Structural Deformation of MTX Induced by Nanodrug Conjugation Dictate Intracellular Drug Transport and Drug Efficacy
Authors: JY Park, JS Hyun, JG Jee, SJ Park, D Khang
International Journal of Nanomedicine, 2021;16(0):4943-4957. 2021
Interleukin-13 rs1800925/-1112C/T promoter single nucleotide polymorphism variant linked to anti-schistosomiasis in adult males in Murehwa District, Zimbabwe
Authors: ET Choto, T Mduluza, MJ Chimbari
PLoS ONE, 2021;16(5):e0252220. 2021
Macrophage migration inhibitory factor may play a protective role in osteoarthritis
Authors: M Liu, Z Xie, G Sun, L Chen, D Qi, H Zhang, J Xiong, A Furey, P Rahman, G Lei, G Zhai
Arthritis Research & Therapy, 2021;23(1):59. 2021
Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model
Authors: V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson
Scientific Reports, 2021;11(1):5227. 2021
Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
Scientific Reports, 2021;11(1):3802. 2021
Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment
Authors: A Essaouiba, R Jellali, M Shinohara, B Scheidecke, C Legallais, Y Sakai, E Leclerc
Journal of biotechnology, 2021;0(0):. 2021
Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies
Authors: M Chatterjee, I van Steeno, E Huisman, L Oosterveld, H Berendse, WM van der Fl, M Del Campo, AW Lemstra, WDJ van de Ber, CE Teunissen
Biomolecules, 2020;10(8):. 2020
Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training
Authors: J Kortas, E Ziemann, D Juszczak, K Micielska, M Koz?owska, K Prusik, K Prusik, J Antosiewic
Nutrients, 2020;12(4):. 2020
Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors
Authors: Z Fan, Y Tian, Z Chen, L Liu, Q Zhou, J He, J Coleman, C Dong, N Li, J Huang, C Xu, Z Zhang, S Gao, P Zhou, K Ding, L Chen
EMBO Mol Med, 2020;0(0):e11571. 2020
Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA Shedding
Authors: X Wu, Y Zhang, Y Li, IGH Schmidt-Wo
Cancers (Basel), 2020;12(7):. 2020
Phase imaging of transition from classical to quantum plasmonic couplings between a metal nanoparticle and a metal surface
Authors: H Wang, H Yu, Y Wang, X Shan, HY Chen, N Tao
Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020
Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor
Authors: ALD Pace, N Tumino, F Besi, C Alicata, LA Conti, E Munari, E Maggi, P Vacca, L Moretta
Cancers (Basel), 2020;12(3):. 2020
Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels
Authors: IM Oliveira, C Gonçalves, ME Shin, S Lee, RL Reis, G Khang, JM Oliveira
Biomolecules, 2020;10(10):. 2020
Differentially expressed circular RNAs in a murine asthma model
Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
Molecular Medicine Reports, 2020;22(6):5412-5422. 2020
Association of PARP1-specific polymorphisms and haplotypes with non-small cell lung cancer subtypes
Authors: J Jin, H Robeson, P Fagan, MS Orloff
PLoS ONE, 2020;15(12):e0243509. 2020
Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
Cells, 2020;9(9):. 2020
Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes
Authors: JAD Van, S Clotet-Fre, AC Hauschild, I Batruch, I Jurisica, Y Elia, FH Mahmud, E Sochett, EP Diamandis, JW Scholey, A Konvalinka
PLoS ONE, 2020;15(5):e0233639. 2020
New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells
Authors: CA Ciocan-Car, A Jurj, O Zanoaga, R Cojocneanu, LA Pop, A Moldovan, C Moldovan, AA Zimta, L Raduly, C Pop-Bica, M Buse, L Budisan, P Virag, A Irimie, SMG Diaz, I Berindan-N, C Braicu
J Exp Clin Cancer Res, 2020;39(1):241. 2020
NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting
Authors: C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman
Nat. Immunol., 2020;0(0):. 2020
Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020;8(1):163. 2020
Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia
Authors: C Wu, J Lu, S Lu, M Huang, Y Xu
J Psychiatr Res, 2020;126(0):92-97. 2020
Resistance to autosomal dominant Alzheimer's disease in an APOE3 Christchurch homozygote: a case report
Authors: JF Arboleda-V, F Lopera, M O'Hare, S Delgado-Ti, C Marino, N Chmielewsk, KL Saez-Torre, D Amarnani, AP Schultz, RA Sperling, D Leyton-Cif, K Chen, A Baena, D Aguillon, S Rios-Romen, M Giraldo, E Guzmán-Vél, DJ Norton, E Pardilla-D, A Artola, JS Sanchez, J Acosta-Uri, M Lalli, KS Kosik, MJ Huentelman, H Zetterberg, K Blennow, RA Reiman, J Luo, Y Chen, P Thiyyagura, Y Su, GR Jun, M Naymik, X Gai, M Bootwalla, J Ji, L Shen, JB Miller, LA Kim, PN Tariot, KA Johnson, EM Reiman, YT Quiroz
Nat. Med., 2019;0(0):. 2019
A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
J Clin Med, 2019;8(10):. 2019
Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
Front Neurosci, 2019;13(0):836. 2019
Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children
Authors: A Posod, R Pechlaner, X Yin, SA Burnap, SJ Kiechl, J Willeit, JL Witztum, M Mayr, S Kiechl, U Kiechl-Koh
J Am Heart Assoc, 2019;8(8):e011199. 2019
Potential relation between soluble growth differentiation factor-15 and testosterone deficiency in male patients with coronary artery disease
Authors: H Liu, Y Lyu, D Li, Y Cui, Y Huang, W Dai, Y Li
Cardiovasc Diabetol, 2019;18(1):21. 2019
Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
Authors: S Pereira, SM O'Dwyer, TD Webber, RK Baker, V So, CE Ellis, JS Yoon, M Mojibian, MM Glavas, S Karunakara, SM Clee, SD Covey, TJ Kieffer
Sci Rep, 2019;9(1):3307. 2019
Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
Cell Stem Cell, 2019;0(0):. 2019
Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
Cell Rep, 2018;25(3):677-689.e4. 2018
Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
Front Immunol, 2018;9(0):742. 2018
Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
Thyroid, 2018;0(0):. 2018
Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStemï¿½-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
Int J Mol Sci, 2018;19(12):. 2018
CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
J. Cell. Mol. Med., 2018;0(0):. 2018
Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
Nat Commun, 2018;9(1):1988. 2018
The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
Transl Psychiatry, 2017;7(4):e1079. 2017
Urinary kallikrein 10 predicts the incurability of gastric cancer
Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
Oncotarget, 2017;8(17):29247-29257. 2017
Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
Sci Rep, 2017;7(1):6871. 2017
Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
Sci Rep, 2017;7(1):365. 2017
Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
Mediators Inflamm., 2017;2017(0):9734837. 2017
Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34ï¿½years of age: a prospective cohort study
Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
Diabetol Metab Syndr, 2017;9(0):41. 2017
Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
J. Biol. Chem., 2017;0(0):. 2017
Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
J Biol Chem, 2016;0(0):. 2016
Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
J Immunol, 0;200(8):2581-2591. 0
Can I use distilled water for diluting the Reagent Diluent Concentrate 2?
R&D systems uses deionized water to dilute Reagent Diluent Concentrate 2. We do not have information on how distilled water may affect the performance.
Supplemental ELISA Products
Reviews for DuoSet ELISA Ancillary Reagent Kit 2
Average Rating: 4.9 (Based on 78 Reviews)
Have you used DuoSet ELISA Ancillary Reagent Kit 2?
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Very good kit. It’s reasonable to buy this kit instead of buying everything separately. The result is reproducible.
This kit is very helpful. The protocol is also easy and straight forward
super convenient and budge friendly
Results are very reproducible and the kit was very helpful and easy to use. This saved a lot of time, with minimal troubleshooting.
The results are reproducible between different Lots of this kit.
Results are reproducible and the kit is easy to follow.
Used for measuring IL2 and IFN-gamma release in T cell cultures.
The DuoSet ELISA Ancillary Reagent Kit 2 was used with the Human TNF-alpha DuoSet ELISA kit (DY210), Human IL-6 DuoSet ELISA (DY206) and Human IL-12/IL-23 p40 DuoSet ELISA (DY124) to detect secreted cytokines in GMCSF/IL4 monocyte-derived human dendritic cells stimulated with LPS (100 ng/ml) for 24 hours.
I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.
The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.
DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.
Used for quantifying release of IL2 and IFN gamma
Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.
Fantastic kit. Very cost effective compared to buying everything separately. Reagent Diluent can be scarce depending on sample protein concentration.
VERY SENSITIVE, GOOD KIT
Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.
On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.
Contains all the reagents needed for the experiments and works well!Well done!
Good standard curve even for linear form
The kit works very well.
we used this kit for developing sandwich ELISA's
Contains all the reagents needed and works well, p.s. nice ladder
The perfect companion to DuoSet ELISA kits, everything you need to run five plates.
I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
Used for measuring NGF in bovine seminal plasma.
The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
Every reagent provided.
This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.
The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.
The box it is provided in is more ergonomic now than its previous versions as well.
This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
Works very well with all compatible DuoSet kits.