DuoSet ELISA Ancillary Reagent Kit 2 Summary
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
- Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
- Clear Polystyrene Microplates (five 12x8-well strip microplates)
- ELISA Plate Sealers (24 plate sealers)
- Color Reagent A (1X, three 12.5 mL vials)
- Color Reagent B (1X, three 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
- Wash Buffer (25X, six 21 mL vials)
Preparation and Storage
Citations for DuoSet ELISA Ancillary Reagent Kit 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
J. Cell. Mol. Med., 2018;0(0):. 2018
Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
Nat Commun, 2018;9(1):1988. 2018
Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
J. Biol. Chem., 2017;0(0):. 2017
Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
Sci Rep, 2017;7(1):365. 2017
Urinary kallikrein 10 predicts the incurability of gastric cancer
Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
Oncotarget, 2017;8(17):29247-29257. 2017
Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
Sci Rep, 2017;7(1):6871. 2017
The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
Transl Psychiatry, 2017;7(4):e1079. 2017
Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
Mediators Inflamm., 2017;2017(0):9734837. 2017
Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34ï¿½years of age: a prospective cohort study
Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
Diabetol Metab Syndr, 2017;9(0):41. 2017
Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
J Biol Chem, 2016;0(0):. 2016
Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
J Immunol, 0;200(8):2581-2591. 0
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Reviews for DuoSet ELISA Ancillary Reagent Kit 2
Average Rating: 4.8 (Based on 31 Reviews)
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I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
Used for measuring NGF in bovine seminal plasma.
The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
Every reagent provided.
This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.
The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.
The box it is provided in is more ergonomic now than its previous versions as well.
This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
Works very well with all compatible DuoSet kits.