DuoSet ELISA Ancillary Reagent Kit 2

Newer Version Available: DY008B
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Product in process of being discontinued and replaced by new Next generation kit

Discontinued Product

DY008 has been discontinued and is replaced by DY008B.

R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (68)
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Reviews (90)

DuoSet ELISA Ancillary Reagent Kit 2 Summary

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays
PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.


PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
  • Wash Buffer (25X, six 21 mL vials)

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for DuoSet ELISA Ancillary Reagent Kit 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

68 Citations: Showing 1 - 10
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  1. VEGF-C serum level is associated with response to bevacizumab maintenance therapy in primary ovarian cancer patients
    Authors: Y Ding, L Oliveira-F, E Vettorazzi, K Legler, K Milde-Lang, L Woelber, A Jaeger, K Prieske, V Mueller, B Schmalfeld, S Kuerti
    PLoS ONE, 2022;17(6):e0269680.  2022
  2. DENV-Mimetic Polymersome Nanoparticles Bearing Multi-Epitope Lipopeptides Antigen as the Next-Generation Dengue Vaccine
    Authors: NA Abdul Rahm, AS Mohamad No, ML Nordin, MCI Mohd Amin, AA Ahmad Fuaa, NA Muhammad A, N Marasini, F Azmi
    Pharmaceutics, 2022;14(1):.  2022
  3. The performance of soluble CD163 as a non-invasive biomarker of liver damage in chronically HCV and HCV/HIV infected subjects
    Authors: V Cairoli, E De Matteo, P Casciato, B Ameigeiras, MV Preciado, P Valva
    PLoS ONE, 2022;17(7):e0270911.  2022
  4. Periostin Augments Vascular Smooth Muscle Cell Calcification via beta-Catenin Signaling
    Authors: I Alesutan, LA Henze, B Boehme, TTD Luong, D Zickler, B Pieske, KU Eckardt, A Pasch, J Voelkl
    Biomolecules, 2022;12(8):.  2022
  5. Association between vascular endothelial growth factor-mediated blood-brain barrier dysfunction and stress-induced depression
    Authors: H Matsuno, S Tsuchimine, K O'Hashi, K Sakai, K Hattori, S Hidese, S Nakajima, S Chiba, A Yoshimura, N Fukuzato, M Kando, M Tatsumi, S Ogawa, N Ichinohe, H Kunugi, K Sohya
    Molecular Psychiatry, 2022;0(0):.  2022
  6. Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Authors: C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente
    Nature Medicine, 2022;28(4):752-765.  2022
  7. Hyaluronidase-1-mediated glycocalyx impairment underlies endothelial abnormalities in polypoidal choroidal vasculopathy
    Authors: KX Wu, NJY Yeo, CY Ng, FWJ Chioh, Q Fan, X Tian, B Yang, G Narayanan, HM Tay, HW Hou, NR Dunn, X Su, CMG Cheung, C Cheung
    Bmc Biology, 2022;20(1):47.  2022
  8. The role of neutrophil elastase in aortic valve calcification
    Authors: Y Liu, P Jiang, L An, M Zhu, J Li, Y Wang, Q Huang, Y Xiang, X Li, Q Shi, Y Weng
    Journal of Translational Medicine, 2022;20(1):167.  2022
  9. Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium
    Authors: P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger
    Cell Reports, 2022;38(13):110571.  2022
  10. Exercise in the heat induces similar elevations in serum irisin in young and older men despite lower resting irisin concentrations in older adults
    Authors: JJ McCormick, KE King, SR Notley, N Fujii, P Boulay, RJ Sigal, GP Kenny
    Journal of thermal biology, 2022;104(0):103189.  2022
  11. Protection against SARS-CoV-2 infection by a mucosal vaccine in rhesus macaques
    Authors: Y Sui, J Li, R Zhang, SK Prabhu, HA Elyard, D Venzon, A Cook, RE Brown, E Teow, J Velasco, J Greenhouse, T Putmon-Tay, TV Campbell, L Pessaint, IN Moore, LA Lagenaur, JD Talton, MW Breed, J Kramer, KW Bock, M Minai, BM Nagata, MG Lewis, LX Wang, JA Berzofsky
    JCI Insight, 2021;0(0):.  2021
  12. NR4A family members regulate T cell tolerance to preserve immune homeostasis and suppress autoimmunity
    Authors: R Hiwa, HV Nielsen, JL Mueller, R Mandla, J Zikherman
    JCI Insight, 2021;0(0):.  2021
  13. Structural Deformation of MTX Induced by Nanodrug Conjugation Dictate Intracellular Drug Transport and Drug Efficacy
    Authors: JY Park, JS Hyun, JG Jee, SJ Park, D Khang
    International Journal of Nanomedicine, 2021;16(0):4943-4957.  2021
  14. Macrophage migration inhibitory factor may play a protective role in osteoarthritis
    Authors: M Liu, Z Xie, G Sun, L Chen, D Qi, H Zhang, J Xiong, A Furey, P Rahman, G Lei, G Zhai
    Arthritis Research & Therapy, 2021;23(1):59.  2021
  15. Interleukin-13 rs1800925/-1112C/T promoter single nucleotide polymorphism variant linked to anti-schistosomiasis in adult males in Murehwa District, Zimbabwe
    Authors: ET Choto, T Mduluza, MJ Chimbari
    PLoS ONE, 2021;16(5):e0252220.  2021
  16. Protein Digests and Pure Peptides from Chia Seed Prevented Adipogenesis and Inflammation by Inhibiting PPAR&gamma and NF-&kappaB Pathways in 3T3L-1 Adipocytes
    Authors: M Grancieri, HSD Martino, E Gonzalez d
    Nutrients, 2021;13(1):.  2021
  17. Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model
    Authors: V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson
    Scientific Reports, 2021;11(1):5227.  2021
  18. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021;11(1):3802.  2021
  19. Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment
    Authors: A Essaouiba, R Jellali, M Shinohara, B Scheidecke, C Legallais, Y Sakai, E Leclerc
    Journal of biotechnology, 2021;0(0):.  2021
  20. Bioengineered Nanoparticles Loaded-Hydrogels to Target TNF Alpha in Inflammatory Diseases
    Authors: IM Oliveira, DC Fernandes, FR Maia, RF Canadas, RL Reis, JM Oliveira
    Pharmaceutics, 2021;13(8):.  2021
  21. Zinc Ameliorates the Osteogenic Effects of High Glucose in Vascular Smooth Muscle Cells
    Authors: LA Henze, M Estepa, B Pieske, F Lang, KU Eckardt, I Alesutan, J Voelkl
    Cells, 2021;10(11):.  2021
  22. Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
    Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
    Molecular Metabolism, 2021;53(0):101336.  2021
  23. Clinical and biological markers for predicting ARDS and outcome in septic patients
    Authors: J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o
    Scientific Reports, 2021;11(1):22702.  2021
  24. Increased Plasma Soluble PD-1 Concentration Correlates with Disease Progression in Patients with Cancer Treated with Anti-PD-1 Antibodies
    Authors: R Ohkuma, K Ieguchi, M Watanabe, D Takayanagi, T Goshima, R Onoue, K Hamada, Y Kubota, A Horiike, T Ishiguro, Y Hirasawa, H Ariizumi, J Tsurutani, K Yoshimura, M Tsuji, Y Kiuchi, S Kobayashi, T Tsunoda, S Wada
    Biomedicines, 2021;9(12):.  2021
  25. Nordic Walking Rather Than High Intensity Interval Training Reduced Myostatin Concentration More Effectively in Elderly Subjects and the Range of This Drop Was Modified by Metabolites of Vitamin D
    Authors: K Micielska, M Flis, JA Kortas, E Rodziewicz, J Antosiewic, K Wochna, G Lombardi, E Ziemann
    Nutrients, 2021;13(12):.  2021
  26. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis
    Authors: JI Wurster, RL Peterson, CE Brown, S Penumutchu, DV Guzior, K Neugebauer, WH Sano, MM Sebastian, RA Quinn, P Belenky
    Cell Reports, 2021;37(11):110113.  2021
  27. NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting
    Authors: C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman
    Nat. Immunol., 2020;0(0):.  2020
  28. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
    Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
    J Immunol Res, 2020;2020(0):9690832.  2020
  29. Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies
    Authors: M Chatterjee, I van Steeno, E Huisman, L Oosterveld, H Berendse, WM van der Fl, M Del Campo, AW Lemstra, WDJ van de Ber, CE Teunissen
    Biomolecules, 2020;10(8):.  2020
  30. Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
    Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
    Cells, 2020;9(9):.  2020
  31. Induction of ASC pyroptosis requires gasdermin D or caspase-1/11-dependent mediators and IFNbeta from pyroptotic macrophages
    Authors: C Zhang, C Zhao, X Chen, R Tao, S Wang, G Meng, X Liu, C Shao, X Su
    Cell Death Dis, 2020;11(6):470.  2020
  32. Topical Administration of SLN-Based Gene Therapy for the Treatment of Corneal Inflammation by De Novo IL-10 Production
    Authors: M Vicente-Pa, I Gómez-Agua, J Rodríguez-, A Rodríguez-, E Muntoni, L Battaglia, A Del Pozo-R, MÁ Solinís As
    Pharmaceutics, 2020;12(6):.  2020
  33. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
    Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
    Acta Neuropathol Commun, 2020;8(1):163.  2020
  34. New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells
    Authors: CA Ciocan-Car, A Jurj, O Zanoaga, R Cojocneanu, LA Pop, A Moldovan, C Moldovan, AA Zimta, L Raduly, C Pop-Bica, M Buse, L Budisan, P Virag, A Irimie, SMG Diaz, I Berindan-N, C Braicu
    J Exp Clin Cancer Res, 2020;39(1):241.  2020
  35. Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training
    Authors: J Kortas, E Ziemann, D Juszczak, K Micielska, M Koz?owska, K Prusik, K Prusik, J Antosiewic
    Nutrients, 2020;12(4):.  2020
  36. Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
    Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
    Cells, 2020;9(9):.  2020
  37. Association of PARP1-specific polymorphisms and haplotypes with non-small cell lung cancer subtypes
    Authors: J Jin, H Robeson, P Fagan, MS Orloff
    PLoS ONE, 2020;15(12):e0243509.  2020
  38. Differentially expressed circular RNAs in a murine asthma model
    Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
    Molecular Medicine Reports, 2020;22(6):5412-5422.  2020
  39. Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies
    Authors: M Chatterjee, I van Steeno, E Huisman, L Oosterveld, H Berendse, WM van der Fl, M Del Campo, AW Lemstra, WDJ van de Ber, CE Teunissen
    Biomolecules, 2020;10(8):.  2020
  40. Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor
    Authors: ALD Pace, N Tumino, F Besi, C Alicata, LA Conti, E Munari, E Maggi, P Vacca, L Moretta
    Cancers (Basel), 2020;12(3):.  2020
  41. Phase imaging of transition from classical to quantum plasmonic couplings between a metal nanoparticle and a metal surface
    Authors: H Wang, H Yu, Y Wang, X Shan, HY Chen, N Tao
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.  2020
  42. Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA Shedding
    Authors: X Wu, Y Zhang, Y Li, IGH Schmidt-Wo
    Cancers (Basel), 2020;12(7):.  2020
  43. Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors
    Authors: Z Fan, Y Tian, Z Chen, L Liu, Q Zhou, J He, J Coleman, C Dong, N Li, J Huang, C Xu, Z Zhang, S Gao, P Zhou, K Ding, L Chen
    EMBO Mol Med, 2020;0(0):e11571.  2020
  44. Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia
    Authors: C Wu, J Lu, S Lu, M Huang, Y Xu
    J Psychiatr Res, 2020;126(0):92-97.  2020
  45. Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes
    Authors: JAD Van, S Clotet-Fre, AC Hauschild, I Batruch, I Jurisica, Y Elia, FH Mahmud, E Sochett, EP Diamandis, JW Scholey, A Konvalinka
    PLoS ONE, 2020;15(5):e0233639.  2020
  46. Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels
    Authors: IM Oliveira, C Gonçalves, ME Shin, S Lee, RL Reis, G Khang, JM Oliveira
    Biomolecules, 2020;10(10):.  2020
  47. Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
    Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
    Front Neurosci, 2019;13(0):836.  2019
  48. Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children
    Authors: A Posod, R Pechlaner, X Yin, SA Burnap, SJ Kiechl, J Willeit, JL Witztum, M Mayr, S Kiechl, U Kiechl-Koh
    J Am Heart Assoc, 2019;8(8):e011199.  2019
  49. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
    Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
    Cell Stem Cell, 2019;0(0):.  2019
  50. A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
    Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
    J Clin Med, 2019;8(10):.  2019
  51. Resistance to autosomal dominant Alzheimer's disease in an APOE3 Christchurch homozygote: a case report
    Authors: JF Arboleda-V, F Lopera, M O'Hare, S Delgado-Ti, C Marino, N Chmielewsk, KL Saez-Torre, D Amarnani, AP Schultz, RA Sperling, D Leyton-Cif, K Chen, A Baena, D Aguillon, S Rios-Romen, M Giraldo, E Guzmán-Vél, DJ Norton, E Pardilla-D, A Artola, JS Sanchez, J Acosta-Uri, M Lalli, KS Kosik, MJ Huentelman, H Zetterberg, K Blennow, RA Reiman, J Luo, Y Chen, P Thiyyagura, Y Su, GR Jun, M Naymik, X Gai, M Bootwalla, J Ji, L Shen, JB Miller, LA Kim, PN Tariot, KA Johnson, EM Reiman, YT Quiroz
    Nat. Med., 2019;0(0):.  2019
  52. Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
    Authors: S Pereira, SM O'Dwyer, TD Webber, RK Baker, V So, CE Ellis, JS Yoon, M Mojibian, MM Glavas, S Karunakara, SM Clee, SD Covey, TJ Kieffer
    Sci Rep, 2019;9(1):3307.  2019
  53. Potential relation between soluble growth differentiation factor-15 and testosterone deficiency in male patients with coronary artery disease
    Authors: H Liu, Y Lyu, D Li, Y Cui, Y Huang, W Dai, Y Li
    Cardiovasc Diabetol, 2019;18(1):21.  2019
  54. CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
    Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
    J. Cell. Mol. Med., 2018;0(0):.  2018
  55. Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
    Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
    Cell Rep, 2018;25(3):677-689.e4.  2018
  56. Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
    Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
    Front Immunol, 2018;9(0):742.  2018
  57. Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
    Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
    Int J Mol Sci, 2018;19(12):.  2018
  58. Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
    Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
    Thyroid, 2018;0(0):.  2018
  59. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
    Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
    Nat Commun, 2018;9(1):1988.  2018
  60. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017;7(4):e1079.  2017
  61. Urinary kallikrein 10 predicts the incurability of gastric cancer
    Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
    Oncotarget, 2017;8(17):29247-29257.  2017
  62. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017;7(1):6871.  2017
  63. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.  2017
  64. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
    Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
    Mediators Inflamm., 2017;2017(0):9734837.  2017
  65. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.  2017
  66. Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
    Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
    J. Biol. Chem., 2017;0(0):.  2017
  67. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016;0(0):.  2016
  68. Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
    Authors: E Lozano, Lozano E, Diaz T, T Díaz, MP Mena, Mena M, Sune G, G Suñe, X Calvo, M Calderón, Calderon M, Perez-Amill L, L Pérez-Amil, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, Rosinol L, L Rosiñol, I Isola, Isola I, Rodriguez-Lobato L, LG Rodríguez-, B Martin-Ant, J Bladé, Blade J, C Fernández
    J Immunol, 0;200(8):2581-2591.  0

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DuoSet ELISA Ancillary Reagent Kit 2
By Gabriele Strusi on 11/21/2022

HepG2 conditioned medium


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/02/2022

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/02/2022

LOW BLANK SIGNAL. HENCE EXCELLENT.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/18/2022

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/31/2022

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/26/2022

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/24/2022

Works very well if protocol is followed. Another high binding plates can also be used optionally


DuoSet ELISA Ancillary Reagent Kit 2
By James Williams on 03/16/2022

Budget friendly and produces excellent results


DuoSet ELISA Ancillary Reagent Kit 2
By Troy Trevino on 03/11/2022

Used with Mouse CXCL10 ELISA Kit DY446


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/16/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/11/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Rob Knight on 12/09/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/06/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/28/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/23/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/19/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/06/2021

Very good kit. It’s reasonable to buy this kit instead of buying everything separately. The result is reproducible.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/06/2021

This kit is very helpful. The protocol is also easy and straight forward


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/18/2021

super convenient and budge friendly


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/09/2021

Results are very reproducible and the kit was very helpful and easy to use. This saved a lot of time, with minimal troubleshooting.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/09/2021

DuoSet ELISA Ancillary Reagent Kit 2
By Arun Kashyap on 12/20/2020

The results are reproducible between different Lots of this kit.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/20/2020

Results are reproducible and the kit is easy to follow.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/18/2020

Used for measuring IL2 and IFN-gamma release in T cell cultures.


DuoSet ELISA Ancillary Reagent Kit 2
By Hon Wai Koon on 12/01/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/28/2020

The DuoSet ELISA Ancillary Reagent Kit 2 was used with the Human TNF-alpha DuoSet ELISA kit (DY210), Human IL-6 DuoSet ELISA (DY206) and Human IL-12/IL-23 p40 DuoSet ELISA (DY124) to detect secreted cytokines in GMCSF/IL4 monocyte-derived human dendritic cells stimulated with LPS (100 ng/ml) for 24 hours.


DuoSet ELISA Ancillary Reagent Kit 2
By APK Inmunometabolismo on 11/06/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Evan Williams on 10/28/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Joseph Ambrose on 10/27/2020

I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/23/2020

Works well!!!


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/23/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/13/2020

The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/13/2020

DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.


DuoSet ELISA Ancillary Reagent Kit 2
By Milton Quintanilla on 10/07/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Pritha Agarwalla on 09/30/2020

Used for quantifying release of IL2 and IFN gamma


DuoSet ELISA Ancillary Reagent Kit 2
By Robert Berahovich on 09/29/2020

Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/14/2020

Fantastic kit. Very cost effective compared to buying everything separately. Reagent Diluent can be scarce depending on sample protein concentration.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/05/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/08/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/17/2020

VERY SENSITIVE, GOOD KIT


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/23/2020

Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.

On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/11/2020

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/14/2019

Contains all the reagents needed for the experiments and works well!Well done!


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/29/2019

Good standard curve even for linear form


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/06/2019

The kit works very well.


DuoSet ELISA Ancillary Reagent Kit 2
By Venkateswarlu Kanamarlapudi on 10/02/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/23/2019

we used this kit for developing sandwich ELISA's


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/21/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/13/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/08/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Gigi Ng on 07/02/2019

Contains all the reagents needed and works well, p.s. nice ladder


DuoSet ELISA Ancillary Reagent Kit 2
By Bulbul Ahmed on 03/30/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Amnon Berger on 03/23/2019

The perfect companion to DuoSet ELISA kits, everything you need to run five plates.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 03/19/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/14/2019

DuoSet ELISA Ancillary Reagent Kit 2
By Ivan Luzardo on 12/11/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/31/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/09/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 09/11/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 08/08/2018

DuoSet ELISA Ancillary Reagent Kit 2
By VICTOR FARRE ALINS on 07/17/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Lucy Quach on 06/21/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Andrada Naghi on 06/20/2018

I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/19/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/04/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/26/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Leslie Priddy on 03/30/2018

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 12/01/2017

DuoSet ELISA Ancillary Reagent Kit 2
By tusar giri on 12/01/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/20/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Maria Yanez on 08/03/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 06/16/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/30/2017

Used for measuring NGF in bovine seminal plasma.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/19/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 05/12/2017

The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA


DuoSet ELISA Ancillary Reagent Kit 2
By reb whitt on 04/25/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 04/12/2017

DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.


DuoSet ELISA Ancillary Reagent Kit 2
By Jonatan Dereke on 01/26/2017

We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.


DuoSet ELISA Ancillary Reagent Kit 2
By Adam Guess on 01/20/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 01/06/2017

DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 11/01/2016

Every reagent provided.


DuoSet ELISA Ancillary Reagent Kit 2
By Anonymous on 10/17/2016

This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.

The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.

The box it is provided in is more ergonomic now than its previous versions as well.


DuoSet ELISA Ancillary Reagent Kit 2
By Jessica Hess on 06/03/2016

This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.


DuoSet ELISA Ancillary Reagent Kit 2
By Jonatan Dereke on 05/19/2016

Works very well with all compatible DuoSet kits.


DuoSet ELISA Ancillary Reagent Kit 2, 5 Plate
By Anonymous on 01/08/2016