Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85277

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dynactin Subunit 2/DCTN2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free (NBP1-85277) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Dynactin Subunit 2/DCTN2/DCTN-50 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Knockdown Validated: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Knockdown-Validated-NBP1-85277-img0017.jpg
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85277-img0016.jpg
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DCTN2 antibody NBP1-85277 (A) shows similar protein distribution across tissues to independent antibody NBP1-85278 (B).
Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Analysis using Anti-DCTN2 antibody NBP1-85277 (A) shows similar pattern to independent antibody NBP1-85278 (B).
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human kidney.
Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human testis.
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human colon.

Applications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF and WB reactivity reported in (PMID: 25669897).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 2/DCTN2

Dynactin subunit 2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Alternate Names

DCTN2, Dynamitin, RBP50

Gene Symbol

DCTN2

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

Citations for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

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Protocols

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