Dysferlin Antibody (CL10888) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43895
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL10888
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of O75923, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Dysferlin Antibody (CL10888) - Azide and BSA Free
![Dysferlin Antibody (CL10888) - Azide and BSA Free Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-282202512182798.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]")
![Dysferlin Antibody (CL10888) - Azide and BSA Free Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-282202513121836.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]")
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]
Staining of human lymph node shows strong membranous positivity in non-germinal center cells and endothelial cells.![Dysferlin Antibody (CL10888) - Azide and BSA Free Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-28220251391966.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]")
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]
Staining of human skin shows no positivity in keratinocytes as expected.![Dysferlin Antibody (CL10888) - Azide and BSA Free Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-28220251331426.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]")
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]
Staining of human placenta shows moderate membranous positivity in trophoblastic cells.![Dysferlin Antibody (CL10888) - Azide and BSA Free Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-282202513415618.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]")
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP3-43895]
Staining of human spleen shows moderate positivity in cells in red pulp.![Dysferlin Antibody (CL10888) - Azide and BSA Free Western Blot: Dysferlin Antibody [NBP3-43895]](https://resources.rndsystems.com/images/products/nbp3-43895_mouse-dysferlin-mab-cl10888-azide-and-bsa-free-28220251324528.jpg "Western Blot: Dysferlin Antibody [NBP3-43895]")
Western Blot: Dysferlin Antibody [NBP3-43895]
Analysis in human cell line RH-30.Applications for Dysferlin Antibody (CL10888) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Dysferlin
Alternate Names
dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B
Gene Symbol
DYSF
Additional Dysferlin Products
Product Documents for Dysferlin Antibody (CL10888) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Dysferlin Antibody (CL10888) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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