Dysferlin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84696

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dysferlin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dysferlin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Dysferlin Antibody [NBP1-84696]

Immunocytochemistry/ Immunofluorescence: Dysferlin Antibody [NBP1-84696]

Immunocytochemistry/Immunofluorescence: Dysferlin Antibody [NBP1-84696] - Staining of human cell line RH-30 shows localization to plasma membrane & microtubule organizing center.
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Analysis in human skeletal muscle and skin tissues. Corresponding Dysferlin RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney, skeletal muscle, skin and testis using Anti-Dysferlin antibody NBP1-84696 (A) shows similar protein distribution across tissues to independent antibody NBP1-84695 (B).
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney shows moderate to strong membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696]

Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skeletal muscle shows strong cytoplasmic and membranous positivity in myocytes.

Applications for Dysferlin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dysferlin

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Alternate Names

dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B

Gene Symbol

DYSF

Additional Dysferlin Products

Product Documents for Dysferlin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Dysferlin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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