E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-54551
![Western Blot: E-Cadherin Antibody (CDH1/1525)Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Western-Blot-NBP2-54551-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525)Azide and BSA Free [NBP2-54551]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CDH1/1525
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Recombinant human E-Cadherin protein (Uniprot: P12830)
Localization
Cell Surface
Marker
Intercellular Junction Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53354).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
Western Blot: E-Cadherin Antibody (CDH1/1525)Azide and BSA Free [NBP2-54551]
Western Blot: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54551-img0008.jpg "Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54551-img0012.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54551-img0001.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54551-img0002.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54551-img0004.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54551-img0009.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).![Protein Array: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-CDH1-1525-Azide-and-BSA-Free-Protein-Array-NBP2-54551-img0007.jpg "Protein Array: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]")
Protein Array: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Protein Array: E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Immunohistochemistry-Paraffin: Mouse Monoclonal E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551]
Immunohistochemistry-Paraffin: Mouse Monoclonal E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free [NBP2-54551] - Immunofluorescent staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP2-54551AF750) in 3% BSA and incubated over night at 4°C. Heat mediated antigen retrieval at pH 6. Image from a verified customer review.
Applications for E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
Product Specific Notices for E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free and earn rewards!
Have you used E-Cadherin Antibody (CDH1/1525) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
