E2F-1 Antibody (JJ092-02)

Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899]

Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.