E4F1 Antibody (4B10) - Azide and BSA Free

Novus Biologicals | Catalog # H00001877-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B10

Format

Azide and BSA Free
View all E4F1 Primary Antibodies »

Product Specifications

Immunogen

E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Specificity

E4F1 - E4F transcription factor 1 (4B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E4F1 Antibody (4B10) - Azide and BSA Free

Western Blot: E4F1 Antibody (4B10) [H00001877-M03]

Western Blot: E4F1 Antibody (4B10) [H00001877-M03]

Western Blot: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysatE (83.5 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03]

Immunocytochemistry/ Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03]

Immunocytochemistry/Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of monoclonal antibody to E4F1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: E4F1 Antibody (4B10) [H00001877-M03] - Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.

Applications for E4F1 Antibody (4B10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E4F1

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. [provided by RefSeq]

Alternate Names

E4F transcription factor 1p50E4F, E4FMGC99614, EC 6.3.2.-, p120E4F, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, transcription factor E4F1

Entrez Gene IDs

1877 (Human)

Gene Symbol

E4F1

UniProt

NP_004415 (Human)

Additional E4F1 Products

Product Documents for E4F1 Antibody (4B10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for E4F1 Antibody (4B10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for E4F1 Antibody (4B10) - Azide and BSA Free

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Protocols

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FAQs

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