E6AP/UBE3A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89092

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all E6AP/UBE3A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E6AP/UBE3A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092]

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092]

Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human testis shows moderate nuclear and cytoplasmic postivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal and glial cells.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic postivity in myocytes.
E6AP/UBE3A Antibody

Western Blot: E6AP/UBE3A Antibody [NBP1-89092] -

Western Blot: E6AP/UBE3A Antibody [NBP1-89092] -Analysis in human cell line RT-4.

Applications for E6AP/UBE3A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E6AP/UBE3A

UBE3A encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Gene Symbol

UBE3A

Additional E6AP/UBE3A Products

Product Documents for E6AP/UBE3A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for E6AP/UBE3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail