EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Novus Biologicals | Catalog # NBP3-16865

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Y4U5 expressed in HEK293
View all EAAT1/GLAST-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EAAT1/GLAST-1/SLC1A3 (P43003). GTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] -

Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Western blot analysis of various lysates using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] -

Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] -

Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] -

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Confocal imaging of paraffin-embedded Rat brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] -

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Confocal imaging of paraffin-embedded Mouse brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EAAT1/GLAST-1

Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2. EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.

Long Name

Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1

Alternate Names

EA6, GLAST-1, GLAST1, SLC1A3

Gene Symbol

SLC1A3

Additional EAAT1/GLAST-1 Products

Product Documents for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)

There are currently no reviews for this product. Be the first to review EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) and earn rewards!

Have you used EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies