EBAG9/RCAS1 Antibody

Novus Biologicals | Catalog # NB100-41096

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EBAG9/RCAS1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KEAQRLMKKEQN corresponding to C-Terminus according to NP_004206.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EBAG9/RCAS1 Antibody

Western Blot: EBAG9/RCAS1 Antibody [NB100-41096]

Western Blot: EBAG9/RCAS1 Antibody [NB100-41096]

Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. (0.2ug/ml)
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for EBAG9/RCAS1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in HEK293 and A431 lysates (calculated MW of 24.4 kDa band according to NP_004206.1and NP_004206.1). A minor band of unknown identity was also consistently observed at 75 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Skeletal Muscle. Use in ICC/IF was reported in scientific literature (PMID: 31433987).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EBAG9/RCAS1

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Entrez Gene IDs

9166 (Human); 55960 (Mouse); 299864 (Rat)

Gene Symbol

EBAG9

UniProt

NP_004206.1 (Human)

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody

Certificate of Analysis

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Product Specific Notices for EBAG9/RCAS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EBAG9/RCAS1 Antibody

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Protocols

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