EBI3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76976

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3. Amino Acid Squence: ILRAVRPRATYYIQV

Specificity

Human EBI3 has only 1 isoform (229aa, 25kD). Mouse EBI3 has one isoform (228aa, 25kD) and rat EBI3 also has one isoform (228aa, 25kD). NBP1-76976 can detect human EBI3 but cannot detect mouse and rat EBI3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EBI3 Antibody - BSA Free

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 Antibody [NBP1-76976] - Analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 ug/mL.
Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 Antibody [NBP1-76976] - K562 lysate with EBI3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
EBI3 Antibody - BSA Free

Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] -

Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.Antibodies: EBI-3, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mL, Lane 2: 2 ug/mL, Lane 3: 4 ug/mL,
EBI3 Antibody - BSA Free

Immunohistochemistry: EBI3 Antibody - BSA Free [NBP1-76976] -

Immunohistochemistry: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
EBI3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EBI3 Antibody - BSA Free [NBP1-76976] -

Immunocytochemistry/ Immunofluorescence: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen tissue labeling EBI-3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for EBI3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

UniProt

NP_005746

Additional EBI3 Products

Product Documents for EBI3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EBI3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

Citations for EBI3 Antibody - BSA Free

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Protocols

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FAQs

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