ECSIT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91858

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ECSIT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECSIT Antibody - BSA Free

Western Blot: ECSIT Antibody [NBP1-91858]

Western Blot: ECSIT Antibody [NBP1-91858]

Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: ECSIT Antibody [NBP1-91858]

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody [NBP1-91858]

Immunocytochemistry/Immunofluorescence: ECSIT Antibody [NBP1-91858] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858]

Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858]

Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Western Blot: ECSIT Antibody [NBP1-91858]

Western Blot: ECSIT Antibody [NBP1-91858]

Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
ECSIT Antibody - BSA Free Western Blot: ECSIT Antibody - BSA Free [NBP1-91858]

Western Blot: ECSIT Antibody - BSA Free [NBP1-91858]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
ECSIT Antibody - BSA Free Western Blot: ECSIT Antibody - BSA Free [NBP1-91858]

Western Blot: ECSIT Antibody - BSA Free [NBP1-91858]

Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for ECSIT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECSIT

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Alternate Names

ECSIT homolog (Drosophila), evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved, Protein SITPEC, signaling intermediate in Toll pathway evolutionarily conserved ortholog, SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Gene Symbol

ECSIT

Additional ECSIT Products

Product Documents for ECSIT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ECSIT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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