eEF-2 Antibody (JE32-57)

Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293]

Western Blot: eEF-2 Antibody (JE32-57) [NBP3-32293] - Western blot analysis of eEF-2 on different lysates with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Jurkat cell lysate (20 ug/Lane)
Lane 3: NIH/3T3 cell lysate (20 ug/Lane)
Lane 4: PC-12 cell lysate (20 ug/Lane)
Lane 5: Mouse liver tissue lysate (40 ug/Lane)
Lane 6: Rat spleen tissue lysate (40 ug/Lane)

Predicted band size: 95 kDa
Observed band size: 95 kDa

Exposure time: 8 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32293) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (JE32-57) [NBP3-32293]

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (JE32-57) [NBP3-32293] - Immunocytochemistry analysis of HeLa cells labeling eEF-2 with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eEF-2 antibody (NBP3-32293) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: eEF-2 Antibody (JE32-57) [NBP3-32293]

Flow Cytometry: eEF-2 Antibody (JE32-57) [NBP3-32293] - Flow cytometric analysis of HeLa cells labeling eEF-2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32293, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).