EEF1G Antibody (2E1O7)

Novus Biologicals | Catalog # NBP3-16694

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E1O7 expressed in HEK293
View all EEF1G Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human EEF1G (P26641). MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1G Antibody (2E1O7)

Western Blot: EEF1G Antibody (2E1O7) [NBP3-16694]

Western Blot: EEF1G Antibody (2E1O7) [NBP3-16694]

Western Blot: EEF1G Antibody (2E1O7) [NBP3-16694] - Western blot analysis of extracts of various cell lines, using EEF1G Rabbit mAb (NBP3-16694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
EEF1G Antibody (2E1O7)

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (2E1O7) [NBP3-16694] -

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (2E1O7) [NBP3-16694] - Immunofluorescence analysis of HeLa cells using EEF1G Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
EEF1G Antibody (2E1O7)

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] -

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] - Immunohistochemistry analysis of EEF1G in paraffin-embedded mouse brain tissue using EEF1G Rabbit mAb at a dilution of 1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1G Antibody (2E1O7)

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] -

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] - Immunohistochemistry analysis of EEF1G in paraffin-embedded human liver tissue using EEF1G Rabbit mAb at a dilution of 1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1G Antibody (2E1O7)

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] -

Immunohistochemistry: EEF1G Antibody (2E1O7) [NBP3-16694] - Immunohistochemistry analysis of EEF1G in paraffin-embedded rat brain tissue using EEF1G Rabbit mAb at a dilution of 1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1G Antibody (2E1O7)

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (2E1O7) [NBP3-16694] -

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (2E1O7) [NBP3-16694] - Immunofluorescence analysis of NIH-3T3 cells using EEF1G Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for EEF1G Antibody (2E1O7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF1G

EEF1G encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Alternate Names

eEF-1B gamma, EF-1-gamma, EF1Gelongation factor 1-gamma, eukaryotic translation elongation factor 1 gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain

Gene Symbol

EEF1G

Additional EEF1G Products

Product Documents for EEF1G Antibody (2E1O7)

Certificate of Analysis

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Product Specific Notices for EEF1G Antibody (2E1O7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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