EEF1G Antibody (3F11-1A10) - Azide and BSA Free

Novus Biologicals | Catalog # H00001937-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F11-1A10

Format

Azide and BSA Free
View all EEF1G Primary Antibodies »

Product Specifications

Immunogen

EEF1G (AAH15813.1, 1 a.a. ~ 437 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYHVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHDKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

EEF1G - eukaryotic translation elongation factor 1 gamma

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Immunocytochemistry/Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of monoclonal antibody to EEF1G on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi ( Cat # H00001937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EEF1G monoclonal antibody (M01), clone 3F11-1A10 (Cat # H00001937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in PC-12.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in HepG2.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in 293.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10 Analysis of EEF1G expression in HL-60.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in Raw 264.7.
Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01]

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in NIH/3T3.

Applications for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EEF1G

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Alternate Names

eEF-1B gamma, EF-1-gamma, EF1Gelongation factor 1-gamma, eukaryotic translation elongation factor 1 gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain

Entrez Gene IDs

1937 (Human)

Gene Symbol

EEF1G

OMIM

130593 (Human)

UniProt

AAH15813 (Human)

Additional EEF1G Products

Product Documents for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

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Protocols

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FAQs

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